Binding of mineral elements to locust bean gum influences availability in vitro

Experiments were conducted to evaluate the extent to which element binding of locust bean gum (LBG) affects the availability of calcium, iron, and zinc in the gut. Infant formula was supplemented with increasing amounts of LBG and subjected to an intraluminal digestion procedure. Element binding was measured by eliminating the complexes by twofold centrifugation. Availability of the elements was determined using a validated continuous-flow dialysis technique. Elemental content of the samples, supernatants, and dialysates was analyzed with validated atomic absorption spectrometry. LBG provided small amounts of intrinsic calcium (1.13 +/- 0.02 mg/g) and trace amounts of iron (0.02 +/- 0.00 mg/g) and zinc (0.01 +/- 0.00 mg/g), which were strongly bound to the LBG molecule (respectively: 76.6 +/- 3.3%, 83.4 +/- 1.2%, 96.7 +/- 6.6%). Correlation analysis, between percent element bound by LBG after centrifugation and percent trapped after dialysis, yielded significant correlation only for the data of zinc (r = 0.93). For calcium and iron, no correlation could be demonstrated, however, for iron a similar trend was observed. These findings suggest that element binding of LBG has a major influence on the availability of zinc and maybe of iron. For calcium, other factors might also be involved, affecting availability.