Molecular cloning and functional expression of the human glycine transporter GlyT2 and chromosomal localisation of the gene in the human genome

被引：63

作者：

Morrow, JA ^{[1
]}

Collie, IT ^{[1
]}

Dunbar, DR ^{[1
]}

Walker, GB ^{[1
]}

Shahid, M ^{[1
]}

Hill, DR ^{[1
]}

机构：

[1] Organon Labs Ltd, Target Discovery Sect, Newhouse ML1 5SH, Lanark, Scotland

来源：

FEBS LETTERS | 1998年 / 439卷 / 03期

关键词：

glycine transporter; cDNA cloning; genomic localization; neurotransmitter; human spinal cord; brain stem; cerebellum;

D O I：

10.1016/S0014-5793(98)01390-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Neurotransmitter transport systems are major targets for therapeutic alterations in synaptic function. We ha ce cloned and sequenced a cDNA encoding the human type 2 glycine transporter GlyT2 from human brain and spinal cord. An open reading frame of 2391 nucleotides encodes a 797 amino acid protein that transports glycine in a Na+/Cl--dependent manner. When stably expressed in CHO cells, human GlyT2 displays a dose-dependent uptake of glycine with an apparent K-m of 108 mu M. This uptake is not affected by sarcosine at concentrations up to 1 mM. Radiation hybrid analysis mapped the GlyT2 gene to D11S1308 (LOD = 8.988) on human chromosome 11p15.1-15.2. (C) 1998 Federation of European Biochemical Societies.

引用

页码：334 / 340

页数：7

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