New antigenic regions of streptokinase are identified by affinity-directed mass spectrometry

被引：11

作者：

Coffey, JA ^{[1
]}

Jennings, KR ^{[1
]}

Dalton, H ^{[1
]}

机构：

[1] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 2001年 / 268卷 / 19期

关键词：

streptokinase; mass spectrometry; affinity-directed mass spectrometry; antigenic regions; epitopes;

D O I：

10.1046/j.0014-2956.2001.02472.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Streptokinase (SK) is a bacterial protein used for the treatment of myocardial infarction, which is immunogenic in humans. Here we report the use of an affinity-directed MS approach to determine the minimal epitopes involved in the binding between SK and patient antibodies. Using this method we have identified two novel epitopes and mapped these to the minimal recognition regions formed by the amino acids D96-E99 and F323-D328. We have also located three previously identified antigenic regions and have now mapped them and shown that they can be defined more precisely as residues P4-L8, P171-P177 and K334-N338.

引用

页码：5215 / 5221

页数：7

共 32 条

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