Bidirectional actions of nociceptin/orphanin FQ on Aδ-fibre-evoked responses in rat superficial spinal dorsal horn in vitro

The present study investigated the modulatory actions of nociceptin/orphanin FQ on excitatory glutamatergic transmission in spinal dorsal horn. In transverse spinal cord slices with an attached dorsal root, mono- and polysynaptic A delta -fibre-evoked extracellular field potentials were recorded from superficial dorsal horn. Nociceptin/orphanin FQ showed bidirectional effects on monosynaptic transmission with a potentiation at lower concentrations (100-300 nM) and a dose-dependent depression at higher concentrations (1-3 muM). The polysynaptic field potential was dose-dependently depressed by nociceptin/orphanin FQ (100 nM-3 muM). None of the actions of nociceptin/orphanin FQ was reversed by the nonspecific opioid receptor antagonist naloxone, the N-methyl-D-aspartate receptor antagonist D-2-amino-5-phosphonovaleric acid or the peptide nocistatin. The bidirectional actions of nociceptin/orphanin FQ on the monosynaptic field potential may provide an in vitro model for the bidirectional actions of nociceptin/orphanin FQ in behavioural studies showing hyperalgesia at low doses of intrathecal nociceptin/orphanin FQ and analgesia at higher doses. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.