Interaction between salsolinol (SAL) and thyrotropin-releasing hormone (TRH) or dopamine (DA) on the secretion of prolactin in ruminants

被引：18

作者：

Hashizume, T. ^{[1
]}

Shida, R. ^{[1
]}

Suzuki, S. ^{[1
]}

Kasuya, E. ^{[2
]}

Kuwayama, H. ^{[3
]}

Suzuki, H. ^{[4
]}

Olah, M. ^{[5
,6
]}

Nagy, G. M. ^{[5
,6
]}

机构：

[1] Iwate Univ, Fac Agr, Morioka, Iwate 0208550, Japan

[2] Natl Inst Agrobiol Sci, Lab Anim Neurophysiol, Tsukuba, Ibaraki 3058550, Japan

[3] Obihiro Univ Agr & Vet Med, Dept Anim Sci, Obihiro, Hokkaido 0800855, Japan

[4] Hirosaki Univ, Fac Agr & Life Sci, Hirosaki, Aomori 0368561, Japan

[5] Hungarian Acad Sci, Dept Human Morphol, Neuromorphol & Neuroendocrine Lab, Budapest, Hungary

[6] Semmelweis Univ, Budapest, Hungary

来源：

DOMESTIC ANIMAL ENDOCRINOLOGY | 2008年 / 34卷 / 03期

基金：

日本学术振兴会;

关键词：

salsolinol; TRH; dopamine; prolactin; ruminants;

D O I：

10.1016/j.domaniend.2007.09.002

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

We have recently demonstrated that salsolinol. (SAL), a dopamine (DA)-derived compound, is present in the posterior pituitary gland and is able to stimulate the release of prolactin (PRL) in ruminants. The aim of the present study was to clarify the effect that the interaction of SAL with thyrotropin-releasing hormone (TRH) or DA has on the secretion of PRL in ruminants. A single intravenous (i.v.) injection of SAL (5 mg/kg body weight (b.w.)),TRH (1 mu g/kg b.w.), and SAL plus TRH significantly stimulated the release of PRL in goats (P < 0.05). The cumulative response curve (area under the curve: AUQ during 120 min was 1.53 and 1.47 times greater after the injection of SAL plus TRH than either SAL or TRH alone, respectively (P < 0.05). A single i.v. injection of sulpiride (a DA receptor antagonist, 0.1 mg/kg b.w.), sulpiride plus SAL (5 mg/kg b.w.), and sulpiride plus TRH (1 mu g/kg b.w.) significantly stimulated the release of PRL in goats (P < 0.05). The AUC of PRL during 120 min was 2.12 and 1.78 times greater after the injection of sulpiride plus TRH than either sulpiride alone or sulpiride plus SAL, respectively (P < 0.05). In cultured bovine anterior pituitary (AP) cells, SAL (10(-6)M), TRH (10(-8) M), and SAL plus TRH significantly increased the release of PRL (P < 0.05), but the additive effect of SAL and TRH detected in vivo was not observed in vitro. In contrast, DA (10(-6) M) inhibited the TRH-, as well as SAL-induced PRL release in vitro. All together, these results clearly show that SAL can stimulate the release of PRL in ruminants. Furthermore, they also demonstrate that the additive effect of SAL and TRE on the release of PRL detected in vivo may not be mediated at the level of the AP, but that DA can overcome their releasing activity both in vivo and in vitro, confirming the dominant role of DA in the inhibitory regulation of PRL secretion in ruminants. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：327 / 332

页数：6

共 15 条

[1] Stress- as well as suckling-induced prolactin release is blocked by a structural analogue of the putative hypophysiotrophic prolactin-releasing factor, salsolinol [J].