In vitro model using mouse hepatocytes for study of alcohol stress

被引：11

作者：

Yang, JW ^{[1
]}

Shin, JS ^{[1
]}

Lee, JJ ^{[1
]}

Chang, HI ^{[1
]}

Kim, CW ^{[1
]}

机构：

[1] Korea Univ, Grad Sch Biotechnol, Seoul 136701, South Korea

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2001年 / 65卷 / 07期

关键词：

mouse hepatocyte; ethanol; allyl alcohol; CYP2E1; hepatotoxicity;

D O I：

10.1271/bbb.65.1528

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, the effects of ethanol and allyl alcohol on primary mouse hepatocytes were investigated. No cytotoxicity was observed by ethanol treatments, but more toxicity to cells was found in the response to allyl alcohol treatment. The expression of cytochrome P450 2E1 (CYP2E1), phase I enzyme was examined in response to ethanol and allyl alcohol. Both xenobiotics induced CYP2E1 up to 1.5 similar to5 fold at the protein level. The effects of insulin on CYP2E1 expression were also measured. Insulin, which has been regarded as an essential hormone for primary hepatocytes, was shown to decrease the level of CYP2E1 protein, and did not affect cell viability. These results on CYP2E1 induction demonstrate that primary mouse hepatocytes, when using ethanol and allyl alcohol as substrates and in insulin-free medium, provide a suitable system for the studies of the role of CYP2E1 in xenobiotic metabolism and toxicity.

引用

页码：1528 / 1533

页数：6

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