4-Oxo-4H-quinolizine-3-carboxylic acids as Mg2+ selective, fluorescent indicators

被引：34

作者：

Otten, PA ^{[1
]}

London, RE ^{[1
]}

Levy, LA ^{[1
]}

机构：

[1] Natl Inst Environm Hlth Sci, Struct Biol Lab, Res Triangle Pk, NC 27709 USA

来源：

BIOCONJUGATE CHEMISTRY | 2001年 / 12卷 / 02期

关键词：

D O I：

10.1021/bc000087d

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper, we present the synthesis of a new series of substituted 4-oxo-4H-quinolizine-3-carboxylic acids and the evaluation of their fluorescent response upon complexation with Mg2+ and Ca2+. This has led to the development of the first Mg2+-selective, ratioable fluorescent indicators. We found that 4-oxo-4H-quinolizine-3-carboxylates are excellent fluorophores which show a strong fluorescent response to Mg2+ but not to Ca-2; the latter metal ion often causing interference during Mg2+ measurements with previously described indicators. The dissociation constants of the studied fluorophores-around 1.0 mM-are of the same order of magnitude as is usually observed for intracellular Mg2+.

引用

页码：203 / 212

页数：10

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