Localized breakdown in linkage disequilibrium does not always predict sperm crossover hot spots in the human MHC class II region

被引：33

作者：

Kauppi, L

Stumpf, MPH

Jeffreys, AJ

机构：

[1] Mem Sloan Kettering Canc Ctr, Program Mol Biol, New York, NY 10021 USA

[2] Univ Leicester, Dept Genet, Leicester LE1 7RH, Leics, England

[3] Univ London Imperial Coll Sci & Technol, Ctr Bioinformat, Div Mol Biosci, London SW7 2AZ, England

来源：

GENOMICS | 2005年 / 86卷 / 01期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

D O I：

10.1016/j.ygeno.2005.03.011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To investigate the relationship between meiotic crossover hot spots and block-like linkage disequilibrium (LD), we have extended our high-resolution studies of the human MHC class II region to a 90-kb segment upstream of the HLA-DOA gene. LD blocks in this region are not as well defined as in the neighboring 210-kb DNA segment but do show two regions of LD breakdown in which coalescent analysis indicates substantial historical recombination. Sperm crossover analysis of one region revealed a novel localized hot spot similar in intensity and morphology to most other MHC hot spots. Crossovers at this hot spot are not obviously affected by a large insertion/deletion polymorphism near the hot spot. The second region of LD breakdown, within the DPB1 gene, shows an extremely low level of sperm crossover activity and does not contain a sperm crossover hot spot. These results highlight the complexity of LD patterns and the importance of experimentally verifying crossover hot spots. (c) 2005 Elsevier Inc. All rights reserved.

引用

收藏

页码：13 / 24

页数：12

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