cDNA cloning of the Cry1Aa receptor variants from Bombyx mori and their expression in mammalian cells

被引：7

作者：

Ikawa, S ^{[1
]}

Tsuda, Y ^{[1
]}

Fukada, T ^{[1
]}

Sugimoto, K ^{[1
]}

Himeno, M ^{[1
]}

机构：

[1] Univ Osaka Prefecture, Dept Appl Biochem, Lab Appl Mol Biol, Sakai, Osaka 5998531, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2000年 / 64卷 / 12期

关键词：

Bacillus thuringiensis; delta-endotoxin; receptor; Bombyx mori; membrane protein;

D O I：

10.1271/bbb.64.2682

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We cloned cDNA of three variants of BtR175, a putative Bombyx mori receptor for Bacillus thuringiensis CrylAa delta -endotoxin by PCR. These variants were likely to be allelic to BtR175, cDNA of BtR175b, the most distant variant from BtR175, was introduced into mammalian cells. BtR175b protein was expressed in the plasma membrane of the cells and showed binding activity to Cry1Aa.

引用

页码：2682 / 2685

页数：4

共 10 条

[1] THE MODE OF ACTION OF BACILLUS-THURINGIENSIS ENDOTOXINS [J].

GILL, SS ;

COWLES, EA ;

PIETRANTONIO, PV .

ANNUAL REVIEW OF ENTOMOLOGY, 1992, 37 :615-636

[2] Purification and partial amino acid sequences of the binding protein from Bombyx mori for CryIAa δ-endotoxin of Bacillus thuringiensis [J].

Ihara, H ;

Uemura, T ;

Masuhara, M ;

Ikawa, S ;

Sugimoto, K ;

Wadano, A ;

Himeno, M .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1998, 120 (01) :197-204

[3] THE RECEPTOR FOR BACILLUS-THURINGIENSIS CRYLA(C) DELTA-ENDOTOXIN IN THE BRUSH-BORDER MEMBRANE OF THE LEPIDOPTERAN MANDUCA-SEXTA IS AMINOPEPTIDASE-N [J].

KNIGHT, PJK ;

CRICKMORE, N ;

ELLAR, DJ .

MOLECULAR MICROBIOLOGY, 1994, 11 (03) :429-436

[4] A 106 kDa form of aminopeptidase is a receptor for Bacillus thuringiensis CryIC delta-endotoxin in the brush border membrane of Manduca sexta [J].

Luo, K ;

Lu, YJ ;

Adang, MJ .

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1996, 26 (8-9) :783-791

[5] Identification of Bombyx mori midgut receptor for Bacillus thuringiensis insecticidal CryIA(a) toxin [J].

Nagamatsu, Y ;

Toda, S ;

Yamaguchi, F ;

Ogo, M ;

Kogure, M ;

Nakamura, M ;

Shibata, Y ;

Katsumoto, T .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1998, 62 (04) :718-726

[6] Cloning, sequencing, and expression of the Bombyx mori receptor for Bacillus thuringiensis insecticidal CryIA(a) toxin [J].

Nagamatsu, Y ;

Toda, S ;

Koike, T ;

Miyoshi, Y ;

Shigematsu, S ;

Kogure, M .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1998, 62 (04) :727-734

[7] The cadherin-like protein is essential to specificity determination and cytotoxic action of the Bacillus thuringiensis insecticidal CryIAa toxin [J].

Nagamatsu, Y ;

Koike, T ;

Sasaki, K ;

Yoshimoto, A ;

Furukawa, Y .

FEBS LETTERS, 1999, 460 (02) :385-390

[8]

RIE JV, 1989, EUR J BIOCHEM, V186, P239

[9] CLONING AND EXPRESSION OF A RECEPTOR FOR AN INSECTICIDAL TOXIN OF BACILLUS-THURINGIENSIS [J].

VADLAMUDI, RK ;

WEBER, E ;

JI, IH ;

JI, TH ;

BULLA, LA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (10) :5490-5494

[10] Aminopeptidase N from Bombyx mori as a candidate for the receptor of Bacillus thuringiensis Cry1Aa toxin [J].

Yaoi, K ;

Kadotani, T ;

Kuwana, H ;

Shinkawa, A ;

Takahashi, T ;

Iwahana, H ;

Sato, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 246 (03) :652-657

← 1 →