Isolation and partial characterization of an opioid-like 88 kDa hibernation-related protein

被引:44
作者
Horton, ND
Kaftani, DJ
Bruce, DS
Bailey, EC
Krober, AS
Jones, JR
Turker, M
Khattar, N
Su, TP
Bolling, SF
Oeltgen, PR
机构
[1] Univ Kentucky, Med Ctr, Dept Pathol, Lexington, KY 40536 USA
[2] Univ Texas, Coll Pharm, Div Pharmacol & Toxicol, Austin, TX USA
[3] Wheaton Coll, Dept Biol, Wheaton, IL USA
[4] NIDA, Portland, OR USA
[5] Univ Michigan, Dept Surg, Ann Arbor, MI 48109 USA
[6] Vet Adm Med Ctr, Lexington, KY 40511 USA
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1998年 / 119卷 / 04期
关键词
hibernation; opioid; HIT; opiate antagonist; glycoprotein; delta opiate receptor; woodchuck; RT-PCR;
D O I
10.1016/S0305-0491(98)00056-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Previous studies show that infusion of hibernating woodchuck albumin (HWA) induces hibernation in summer-active ground squirrels and results in profound behavioral and physiological depression in primates. These effects are reversed by the administration of opiate antagonists, suggesting that the putative hibernation induction trigger (HIT) may act through opioid receptors. We have demonstrated that both HIT-containing plasma and the synthetic alpha opioid D-Ala(2)-D-Leu(5)-enkephalin (DADLE), which mimics the activity of HIT in hibernators, extend tissue survival time of a multi-organ autoperfusion system by 3-fold. In this study we present the first data showing biological activity with a much more highly purified plasma fraction from hibernating woodchucks, identified as the hibernation-related factor (HRF). Both the HRF and DADLE show opiate-like contractile inhibition in the mouse vas deferens (Mvd) bioassay. We also have preliminary evidence in an isolated rabbit heart preparation indicating that the HRF and DADLE act similarly to restore left ventricular function following global myocardial ischemia. Furthermore, we have partially sequenced an alpha 1-glycoprotein-like 88 kDa hibernation-related protein (p88 HRP) present in this fraction, which may prove to be the blood-borne HIT molecule. (C) 1998 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:787 / 805
页数:19
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