Sensitivity enhancement in the TROSY experiment

A modification of the TROSY experiment which allows for sensitivity enhancement without introduction of additional delays is presented. The method relies on the combination of two orthogonal pathways and results in a signal-to-noise gain of root 2 independent of the size of the molecule. The sensitivity enhanced TROSY scheme can be combined with gradient coherence selection. The method has been applied to the 269 residue serine protease PB92. (C) 1998 Academic Press.