Hemoglobin is expressed in alveolar epithelial type II cells

被引：51

作者：

Bhaskaran, M ^{[1
]}

Chen, HF ^{[1
]}

Chen, ZM ^{[1
]}

Liu, L ^{[1
]}

机构：

[1] Oklahoma State Univ, Coll Vet Med, Dept Physiol Sci, Stillwater, OK 74078 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 333卷 / 04期

关键词：

hemoglobin; trans-differentiation; alveolar epithelial cells;

D O I：

10.1016/j.bbrc.2005.06.042

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hemoglobin is the main oxygen carrying heme protein in erythrocytes. In an effort to study the differential gene expression of alveolar epithelial type I and type II cells using DNA microarray technique, we found that the mRNAs of hemoglobin alpha- and beta-chains were expressed in type II cells, but not in type I cells. The microarray data were confirmed by RT-PCR. The mRNA expression of both chains decreased when type II cells trans-differentiated into type I-like cells. Immunocyto/histochemistry revealed that hemoglobin protein was specifically localized in type II cells of a lung cell mixture and rat lung tissue. The endogenous synthesis of hemoglobin in alveolar epithelial cells suggests that hemoglobin may have unidentified functions other than oxygen transport in the lung. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：1348 / 1352

页数：5

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