A transient RNA interference assay system using Arabidopsis protoplasts

被引：22

作者：

An, CI ^{[1
]}

Sawada, A ^{[1
]}

Fukusaki, E ^{[1
]}

Kobayashi, A ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Engn, Dept Biotechnol, Suita, Osaka 5650871, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2003年 / 67卷 / 12期

基金：

日本学术振兴会;

关键词：

RNA interference; double-stranded RNA; Arabidopsis thaliana; protoplasts;

D O I：

10.1271/bbb.67.2674

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Introduction of dsRNA into protoplasts led to marked silencing of target transgenes. Our assay system would provide a convenient and efficient way to induce RNAi in protoplasts of the model plant Arabidopsis thaliana.

引用

页码：2674 / 2677

页数：4

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