Molecular mapping of the rf1 gene for pollen fertility restoration in sorghum (Sorghum bicolor L.)

被引：33

作者：

Klein, RR ^{[1
]}

Klein, PE

Chhabra, AK

Dong, J

Pammi, S

Childs, KL

Mullet, JE

Rooney, WL

Schertz, KF

机构：

[1] ARS, USDA, So Plains Agr Res Ctr, College Stn, TX 77845 USA

[2] Texas A&M Univ, Dept Biochem Biophys, College Stn, TX 77843 USA

[3] Texas A&M Univ, Inst Plant Genom & Biotechnol, College Stn, TX 77843 USA

[4] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA

来源：

THEORETICAL AND APPLIED GENETICS | 2001年 / 102卷 / 08期

关键词：

sorghum (Sorghum bicolor L.); fertility restoration; AFLP; microsatellite;

D O I：

10.1007/s001220100575

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

We report the molecular mapping of a gene for pollen fertility in Al (milo) type cytoplasm of sorghum using AFLP and SSR marker analysis. DNA from an F-2 population comprised of 83 individuals was screened with AFLP genetic markers to detect polymorphic DNAs linked to fertility restoration. Fifteen AFLP markers were linked to fertility restoration from the initial screening with 49 unique AFLP primer combinations (+3/+3 selective bases). As many of these AFLP markers had been previously mapped to a high-density genetic map of sorghum, the,target gene (rf1) could be mapped to linkage group H. Confirmation of the map location of rf1 was obtained by demonstrating that additional linkage group-H markers (SSR, STS, AFLP) were linked to fertility restoration. The closest marker, AFLP Xtsa2582, mapped within 2.4 cM of the target loci while two SSRs, Xtxp18 and Xtxp250, flanked the rf1 locus at 12 cM and 10.8 cM, respectively. The availability of molecular markers will facilitate the selection of pollen fertility restoration in sorghum inbred-line development and provide the foundation for map-based gene isolation.

引用

页码：1206 / 1212

页数：7

共 34 条

[1]

[Anonymous], 1989, PROCEED GRAIN SORGHU, DOI [DOI 10.2135/CROPSCI1978.0011183X001800050055X, 10.2135/cropsci1978.0011183X001800050055x]

[2] The unified grass genome: Synergy in synteny [J].

Bennetzen, JL ;

Freeling, M .

GENOME RESEARCH, 1997, 7 (04) :301-306

[3] Grass genomes [J].

Bennetzen, JL ;

SanMiguel, P ;

Chen, MS ;

Tikhonov, A ;

Francki, M ;

Avramova, Z .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (05) :1975-1978

[4] Genetics and molecular mapping of a male fertility restoration locus (Rfg1) in rye (Secale cereale L.) [J].

Börner, A ;

Korzun, V ;

Polley, A ;

Malyshev, S ;

Melz, G .

THEORETICAL AND APPLIED GENETICS, 1998, 97 (1-2) :99-102

[5] The rf2 nuclear restorer gene of male-sterile T-cytoplasm maize [J].

Cui, XQ ;

Wise, RP ;

Schnable, PS .

SCIENCE, 1996, 272 (5266) :1334-1336

[6]

ERICHSEN A. W., 1963, CROP SCI, V3, P481, DOI 10.2135/cropsci1963.0011183X000300060007x

[7] Comparative genetics in the grasses [J].

Gale, MD ;

Devos, KM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (05) :1971-1974

[8] Plant comparative genetics after 10 years [J].

Gale, MD ;

Devos, KM .

SCIENCE, 1998, 282 (5389) :656-659

[9] POLLEN FERTILITY RESTORATION BY NUCLEAR GENE FR IN CMS COMMON BEAN - AN FR LINKAGE MAP AND THE MODE OF FR ACTION [J].

HE, S ;

YU, ZH ;

VALLEJOS, CE ;

MACKENZIE, SA .

THEORETICAL AND APPLIED GENETICS, 1995, 90 (7-8) :1056-1062

[10] A rapid PCR-aided selection of a rice line containing the Rf-1 gene which is involved in restoration of the cytoplasmic male sterility [J].

Ichikawa, N ;

Kishimoto, N ;

Inagaki, A ;

Nakamura, A ;

Koshino, Y ;

Yokozeki, Y ;

Oka, M ;

Samoto, S ;

Akagi, H ;

Higo, K ;

Shinjyo, C ;

Fujimura, T ;

Shimada, H .

MOLECULAR BREEDING, 1997, 3 (03) :195-202

← 1 2 3 4 →