Suppression of spontaneous and hydrogen peroxide-induced mutations by a MutT-type nucleotide pool sanitization enzyme, the Escherichia coli Orf135 protein

被引：37

作者：

Kamiya, H

Iida, E

Murata-Kamiya, N

Yamamoto, Y

Miki, T

Harashima, H

机构：

[1] Hokkaido Univ, Grad Sch Pharmaceut Sci, Kita Ku, Sapporo, Hokkaido 0600812, Japan

[2] Hokkaido Univ, Inst Med Genet, Kita Ku, Sapporo, Hokkaido 0600815, Japan

[3] Hyogo Med Univ, Nishinomiya, Hyogo 6638501, Japan

[4] Fukuoka Dent Coll, Sawara Ku, Fukuoka 8140193, Japan

来源：

GENES TO CELLS | 2003年 / 8卷 / 12期

关键词：

D O I：

10.1046/j.1365-2443.2003.00688.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background: We recently found that the Escherichia coli Orf135 protein, a MutT-type enzyme, hydrolysed 2-hydroxy-dATP (2-OH-dATP), and less efficiently, 8-hydroxy-dGTP. Results: In this study, we examined the effects of the absence of the orf135 gene. Frequencies of spontaneous and H2O2-induced mutations were two- to three-fold higher in the orf135(-) strain than in the wild-type strain. These mutations include various mutations involving a G:C-->T:A transversion, the same type of mutation elicited by 2-OH-dATP. Over-expression of the Orf135 protein suppressed mutations even in the wild-type strain, as well as in the orf135(-) strain. Conclusions: The mutator phenotype of bacteria lacking the Orf135 protein suggests that this protein is involved in the suppression of mutations induced by oxidized deoxynucleotides in vivo and that various MutT-type enzymes contribute to nucleotide pool sanitization.

引用

页码：941 / 950

页数：10