SwellGel: An affinity chromatography technology for high-capacity and high-throughput purification of recombinant-tagged proteins

被引：14

作者：

Draveling, C ^{[1
]}

Ren, L ^{[1
]}

Haney, P ^{[1
]}

Zeisse, D ^{[1
]}

Qoronfleh, MW ^{[1
]}

机构：

[1] Perbio Sci, Milwaukee, WI 53202 USA

来源：

PROTEIN EXPRESSION AND PURIFICATION | 2001年 / 22卷 / 02期

关键词：

D O I：

10.1006/prep.2001.1462

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The revolution in genomics and proteomics is having a profound impact on drug discovery. Today's protein scientist demands a faster, easier, more reliable way to purify proteins. A high capacity, high-throughput new technology has been developed in Perbio Sciences for affinity protein purification. This technology utilizes selected chromatography media that are dehydrated to form uniform aggregates. The SwellGel aggregates will instantly rehydrate upon addition of the protein sample, allowing purification and direct performance of multiple assays in a variety of formats. SwellGel technology has greater stability and is easier to handle than standard wet chromatography resins. The microplate format of this technology provides high-capacity, high-throughput features, recovering milligram quantities of protein suitable for high-throughput screening or biophysical/structural studies. Data will be presented applying SwellGel technology to recombinant 6x His-tagged protein and glutathione-S-transferase (GST) fusion protein purification. (C) 2001 Academic Press.

引用

页码：359 / 366

页数：8

共 24 条

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