Comparison of one-dimensional and comprehensive two-dimensional separations by gas chromatography

被引:91
作者
Blumberg, Leonid M. [1 ]
David, Frank [2 ]
Klee, Matthew S. [3 ]
Sandra, Pat [2 ]
机构
[1] Fast GC Consulting, Wilmington, DE 19801 USA
[2] RIC, B-8500 Kortrijk, Belgium
[3] Agilent Technol, Wilmington, DE 19808 USA
关键词
1D-GC; comprehensive GCxGC; general separation performance; modulation; optimization; peak capacity; selectivity; separation capacity;
D O I
10.1016/j.chroma.2008.02.044
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Comprehensive two-dimensional gas chromatography (GC x GC) can reveal information on the composition of a sample in a way that cannot be done by one-dimensional GC (1D-GC). GCxGC also offers much greater control of chromatographic selectivity based on molecular structure. However, in spite of more than 15 years of claims of the ability of GCxGC to resolve an overwhelmingly larger number of peaks than ID-GC, and in spite of the theoretically proven potential of GCxGC to have an order of magnitude larger peak capacity than ID-GC, the peak capacity of currently practiced GCxGC does not generally exceed the peak capacity attainable from ID-GC with the same analysis time and the same minimal detectable concentration (MDC). The methodology for comparing the peak capacity of GCxGC to ID-GC is described. The comparison of the performance of GCxGC to ID-GC shows that the modulator is the key bottleneck limiting the performance of existing GCxGC. To realize the full potential of GC x GC, duration of injection from a modulator into the second-dimension column should be reduced by an order of magnitude or more. Use of powerful data analysis techniques such as peak deconvolution in both dimensions can further increase resolving power of GC x GC. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:2 / 16
页数:15
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