Biomedical soft contact-lens sensor for in situ ocular biomonitoring of tear contents

被引：58

作者：

Chu, MingXing ^{[2
]}

Shirai, Takayuki ^{[3
]}

Takahashi, Daishi ^{[1
]}

Arakawa, Takahiro ^{[1
]}

Kudo, Hiroyuki ^{[1
]}

Sano, Kenji ^{[4
]}

Sawada, Shin-ichi ^{[5
]}

Yano, Kazuyoshi ^{[3
]}

Iwasaki, Yasuhiko ^{[6
]}

Akiyoshi, Kazunari ^{[5
]}

Mochizuki, Manabu ^{[4
]}

Mitsubayashi, Kohji ^{[1
,2
]}

机构：

[1] Tokyo Med & Dent Univ, Dept Biomed Devices & Instrumentat, Inst Biomat & Bioengn, Chiyoda Ku, Tokyo 1010062, Japan

[2] Tokyo Med & Dent Univ, Grad Sch Med & Dent Sci, Dept Adv Sci & Technol Biomed Sensors, Bunkyo Ku, Tokyo 1138549, Japan

[3] Tokyo Univ Technol, Grad Sch Bion, Tokyo 1920982, Japan

[4] Tokyo Med & Dent Univ, Dept Ophthalmol & Visual Sci, Bunkyo Ku, Tokyo 1318519, Japan

[5] Tokyo Med & Dent Univ, Dept Organ Mat, Inst Biomat & Bioengn, Chiyoda Ku, Tokyo 1010062, Japan

[6] Kansai Univ, Dept Chem & Mat Engn, Fac Chem Mat & Bioengn, Suita, Osaka 5648680, Japan

来源：

BIOMEDICAL MICRODEVICES | 2011年 / 13卷 / 04期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

Contact-lens; Biosensor; Non-invasive; Ocular biomonitoring; Glucose; EPIDERMAL-GROWTH-FACTOR; RHEUMATOID-ARTHRITIS; SJOGRENS-SYNDROME; GLUCOSE; FLUID; CONJUNCTIVA; ELECTRONICS; ANESTHESIA; BIOSENSOR; DISEASE;

D O I：

10.1007/s10544-011-9530-x

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A soft contact-lens biosensor (SCL-biosensor) for novel non-invasive biomonitoring of tear fluids was fabricated and tested. Wearing a biosensor on eye enabled the in situ monitoring of tear contents. The biosensor has an enzyme immobilized electrode on the surface of a polydimethyl siloxane (PDMS) contact lens. The SCL-biosensor was fabricated using microfabrication techniques for functional polymers (PDMS and 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer). In investigation of in vitro characterization, the SCL-biosensor showed excellent relationship between the output current and glucose concentration from 0.03 to 5.0 mmol center dot L-1, with a correlation coefficient of 0.994. The calibration range covered the reported tear glucose concentrations (0.14 mmol center dot L-1). Based on the result, ocular biomonitoring with the SCL-biosensor was carried out. The SCL-biosensor well worked both in the static state and the dynamic state. The tear glucose level of rabbit was estimated to 0.12 mmol center dot L-1 at first and then the tear turnover was successfully calculated to be 29.6 A +/- 8.42% min(-1). The result indicated that SCL-biosensor is useful for advanced biomonitoring on eye.

引用

页码：603 / 611

页数：9

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