Nanometer arrays of functional light harvesting antenna complexes by nanoimprint lithography and host-guest interactions

被引:66
作者
Escalante, Maryana [2 ]
Zhao, Yiping [1 ,3 ]
Ludden, Marlon J. W. [1 ]
Vermeij, Rolf [2 ]
Olsen, John D. [4 ]
Berenschot, Erwin [3 ]
Hunter, C. Neil [4 ]
Huskens, Jurriaan [1 ]
Subramaniam, Vinod [2 ]
Otto, Cees [2 ]
机构
[1] Univ Twente, Mol Nanofabricat Grp, NL-7500 AE Enschede, Netherlands
[2] Univ Twente, Biophys Engn Grp, NL-7500 AE Enschede, Netherlands
[3] Univ Twente, Transducer Sci & Technol Grp, NL-7500 AE Enschede, Netherlands
[4] Univ Sheffield, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, England
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1021/ja802843m
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We show an approach based on combination of site-directed mutagenesis, NIL and multivalent host-guest interactions for the realization of engineered ordered functional arrays of purified components of the photosynthetic system, the membrane-bound LH2 complex. In addition to micrometer-scale patterned structures, we demonstrated the use of nanometer-scale hard NIL stamps to generate functional protein arrays approaching molecular dimensions.
引用
收藏
页码:8892 / +
页数:3
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