共 15 条
Nanometer arrays of functional light harvesting antenna complexes by nanoimprint lithography and host-guest interactions
被引:66
作者:
Escalante, Maryana
[2
]
Zhao, Yiping
[1
,3
]
Ludden, Marlon J. W.
[1
]
Vermeij, Rolf
[2
]
Olsen, John D.
[4
]
Berenschot, Erwin
[3
]
Hunter, C. Neil
[4
]
Huskens, Jurriaan
[1
]
Subramaniam, Vinod
[2
]
Otto, Cees
[2
]
机构:
[1] Univ Twente, Mol Nanofabricat Grp, NL-7500 AE Enschede, Netherlands
[2] Univ Twente, Biophys Engn Grp, NL-7500 AE Enschede, Netherlands
[3] Univ Twente, Transducer Sci & Technol Grp, NL-7500 AE Enschede, Netherlands
[4] Univ Sheffield, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, England
基金:
英国生物技术与生命科学研究理事会;
关键词:
D O I:
10.1021/ja802843m
中图分类号:
O6 [化学];
学科分类号:
0703 ;
摘要:
We show an approach based on combination of site-directed mutagenesis, NIL and multivalent host-guest interactions for the realization of engineered ordered functional arrays of purified components of the photosynthetic system, the membrane-bound LH2 complex. In addition to micrometer-scale patterned structures, we demonstrated the use of nanometer-scale hard NIL stamps to generate functional protein arrays approaching molecular dimensions.
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页码:8892 / +
页数:3
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