Signature-tagged mutagenesis of Pasteurella multocida identifies mutants displaying differential virulence characteristics in mice and chickens

被引：49

作者：

Harper, M

Boyce, JD

Wilkie, IW

Adler, B ^{[1
]}

机构：

[1] Monash Univ, Dept Microbiol, Bacterial Pathogenesis Res Grp, Clayton, Vic 3800, Australia

[2] Univ Queensland, Brisbane, Qld 4072, Australia

来源：

INFECTION AND IMMUNITY | 2003年 / 71卷 / 09期

关键词：

D O I：

10.1128/IAI.71.9.5440-5446.2003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Pasteurella multocida is the causative agent of fowl cholera in birds. Signature-tagged mutagenesis (STM) was used to identify potential virulence factors in a mouse septicemia disease model and a chicken fowl cholera model. A library of P. multocida mutants was constructed with a modified Tn916 and screened for attenuation in both animal models. Mutants identified by the STM screening were confirmed as attenuated by competitive growth assays in both chickens and mice. Of the 15 mutants identified in the chicken model, only 5 were also attenuated in mice, showing for the first time the presence of host-specific virulence factors and indicating the importance of screening for attenuation in the natural host.

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收藏

页码：5440 / 5446

页数：7

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