CpG motif-based adjuvant as a replacement for Freund's complete adjuvant in a recombinant LHRH vaccine

被引：19

作者：

Conforti, Valeria A. ^{[1
]}

De Avila, David M. ^{[1
]}

Curnmings, Nada S. ^{[1
]}

Zanella, Ricardo ^{[1
]}

Wells, Kenny J. ^{[1
]}

Uelker, Hasan ^{[2
]}

Reeves, Jerry J. ^{[1
]}

机构：

[1] Washington State Univ, Dept Anim Sci, Pullman, WA 99164 USA

[2] Yuzuncu Yil Univ, Ziraat Fac, Dept Anim Sci, TR-65080 Van, Turkey

来源：

VACCINE | 2008年 / 26卷 / 07期

关键词：

LHRH immunization; CpG ODN; Freund's adjuvant;

D O I：

10.1016/j.vaccine.2007.12.010

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

This study compared: (1) Freund's complete adjuvant and CpG oligodeoxynucleotide (ODN) 2006 in water-in-oil emulsion as adjuvants; and (2) increasing doses of a recombinant ovalbumin-LHRH (ova-LHRH) fusion protein as an antigen for a contraceptive vaccine. Treatment groups (n = 8 heifers/group) were: one untreated control group; five groups receiving CpG ODN with different doses of ova-LHRH (1.5; 2.3; 3.4; 5.1; and 7.6 mg); and one group receiving 3.4 mg ova-LHRH in Freund's. Heifers were immunized at weeks 0 and 14. Ail vaccine treatments caused gonadal regression and estrus suppression. CpG ODN is a suitable replacement for Freund's for LHRH immunization. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：907 / 913

页数：7

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