The effectiveness of a CpG motif-based adjuvant (CpG ODN 2006) for LHRH immunization

被引：16

作者：

Conforti, Valeria A. ^{[1
]}

de Avila, David M.

Cummings, Nada S.

Wells, Kenny J.

Uelker, Hasan

Reeves, Jeffy J.

机构：

[1] Washington State Univ, Dept Anim Sci, Pullman, WA 99164 USA

[2] Yuzunci Yil Univ, Ziraat Fak, Dept Anim Sci, TR-65080 Van, Turkey

来源：

VACCINE | 2007年 / 25卷 / 35期

关键词：

LHRH immunization; CpG ODN; Alycobacterium butyricum;

D O I：

10.1016/j.vaccine.2007.05.056

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A recombinant ovalbumin-luteinizing hormone-releasing hormone (ova-LHRH) antigen has been developed for immunocontraception. In this study, a novel immunostimulant for ova-LHRH immunization, CpG oligodeoxynucleotide (ODN) 2006, was compared against Mycobacterium butyricum. Also, the immunogenicity of ova-LHRH after lyophilization and exposure to organic solvents was assessed. Rats received either ova-LHRH solubilized in urea; lyophilized ova-LHRH; lyophilized ova-LHRH exposed to methylene chloride; or lyophilized ova-LHRH exposed to ethyl acetate. Immunogenicity of lyophilized ova-LHRH was reduced compared with solubilized ova-LHRH. Exposure to ethyl acetate further decreased immunogenicity of ova-LHRH. CpG ODN 2006 was a more effective immunostimulant than M. butyricum for LHRH immunization. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：6537 / 6543

页数：7

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