The limiting mobility of DNA sequencing fragments for both cross-linked and noncross-linked polymers in capillary electrophoresis: DNA sequencing at 1200 V cm(-1)

被引：35

作者：

Yan, JY ^{[1
]}

Best, N ^{[1
]}

Zhang, JZ ^{[1
]}

Ren, HJ ^{[1
]}

Jiang, R ^{[1
]}

Hou, J ^{[1
]}

Dovichi, NJ ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT CHEM,EDMONTON,AB T6G 2G2,CANADA

来源：

ELECTROPHORESIS | 1996年 / 17卷 / 06期

关键词：

capillary electrophoresis; DNA sequencing;

D O I：

10.1002/elps.1150170611

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The mobility of DNA sequencing fragments was measured in Long-Ranger gels at an electric field ranging from 200 to 1200 V cm(-1) and in noncross-linked polyacrylamide at electric fields ranging from 100 to 300 V cm(-1). In both cases, N* the fragment length that denotes the onset of biased reptation with orientation, is inversely proportional to electric field. The inverse dependence of N* is inconsistent with the original biased reptation model but is consistent with modern models of DNA migration. While separation speed increases dramatically with electric field, the number of bases determined in a separation decreases in proportion to field strength. We present a DNA sequencing run at an electric field of 1200 V cm(-1). Roughly 200 bases of sequence are determined in 3.5 min.

引用

页码：1037 / 1045

页数：9

共 37 条

[21] ACTIVATION-ENERGY OF SINGLE-STRANDED-DNA MOVING THROUGH CROSS-LINKED POLYACRYLAMIDE GELS AT 300-V/CM - EFFECT OF TEMPERATURE ON SEQUENCING RATE IN HIGH-ELECTRIC-FIELD CAPILLARY GEL-ELECTROPHORESIS [J].