Differentiation of neuroblastoma cell line N1E-115 involves several signaling cascades

被引：12

作者：

Oh, J ^{[1
]}

Karlmark, K ^{[1
]}

Shin, J ^{[1
]}

Pollak, A ^{[1
]}

Freilinger, A ^{[1
]}

Hengstschläger, M ^{[1
]}

Lubec, G ^{[1
]}

机构：

[1] Med Univ Vienna, Dept Pediat, A-1090 Vienna, Austria

来源：

NEUROCHEMICAL RESEARCH | 2005年 / 30卷 / 03期

关键词：

signaling cascades; GTP-binding; Ras-related proteins; kinases; growth factors; calcium binding proteins; phosphatase; NO signaling proteins; retinoic acid-binding protein; differentiation; N1E-115; two-dimensional gel electrophoresis; matrix assisted laser desorption; ionization-time of flight mass spectrometry (MALDI-TOF MS);

D O I：

10.1007/s11064-005-2607-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

No systematic searches for differential expression of signaling proteins (SP) in undifferentiated vs. differentiated cell lineages were published and herein we used protein profiling for this purpose. The N1E-115 cell line was cultivated and an aliquot was differentiated with dimethylsulfoxide (DMSO), that is known to lead to a neuronal phenotype. Cell lysates were prepared, run on two-dimensional gel electrophoresis followed by MALDI-TOF-TOF identification of proteins and maps of identified SPs were generated. Seven SPs were comparable, 27 SPs: GTP-binding/Ras-related proteins, kinases, growth factors, calcium binding proteins, phosphatase-related proteins were observed in differentiated N1E-115 cells and eight SPs of the groups mentioned above were observed in undifferentiated cells only. Switching-on/off of several individual SPs from different signaling cascades during the differentiation process is a key to understand mechanisms involved. The findings reported herein are challenging in vitro and in vivo studies to confirm a functional role for deranged SPs.

引用

页码：333 / 348

页数：16

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