Isolation and characterization of the murine Nanog gene promoter

被引：41

作者：

Wu, DY ^{[1
]}

Yao, Z ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Stem Cell Biol,Lab Mol Cell Biol, Shanghai 200031, Peoples R China

来源：

CELL RESEARCH | 2005年 / 15卷 / 05期

关键词：

Nanog; promoter; F9 EC cells; Oct-1; Oct-4; Oct-6;

D O I：

10.1038/sj.cr.7290300

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Nanog protein is expressed in the interior cells of compacted morulae and maintained till epiblasts but downregulated by implantation stage. It is also expressed in embryonic stem cells, embryonic carcinoma cells and embryonic germ cells but disappeared in differentiated ES cells. In this study, we have isolated, sequenced, and performed the first characterization of the Nanog promoter. The transcription start sites were mapped by primer extension analysis. Two promoter regions were found upstream the transcription start sites and the expression of major Nanog promoter/reporter gene construct is abolished in differentiated F9 EC cells as compared to the undifferentiated counterpart. We also showed that a putative octamer motif (ATGCAAAA) is necessary for the major promoter activity. Gel shift and supershift assays showed that Oct-1, Oct-4 and Oct-6 protein selectively bind to the octamer motif.

引用

页码：317 / 324

页数：8

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