SCAPE:: A new tool for the selective CApture of PEptides in protein identification

被引：22

作者：

Betancourt, L ^{[1
]}

Gil, J ^{[1
]}

Besada, V ^{[1
]}

González, LJ ^{[1
]}

Fernández-de-Cossio, J ^{[1
]}

García, L ^{[1
]}

Pajón, R ^{[1
]}

Sanchez, A ^{[1
]}

Alvarez, F ^{[1
]}

Padrón, G ^{[1
]}

机构：

[1] Ctr Genet Engn & Biotechnol, Dept Proteome Anal, Havana, Cuba

来源：

JOURNAL OF PROTEOME RESEARCH | 2005年 / 4卷 / 02期

关键词：

proteomics; selective-peptide-isolation; protein-identification; cation-exchange; simplification; mass-spectrometry;

D O I：

10.1021/pr049794x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the selective capture and analysis of peptides containing neither histidine nor arginine is evaluated. It is based on the reversible modification of alpha- and epsilon-amino groups of peptides and the relatively easy separation of charged and noncharged peptides by cation exchange chromatography. The simplicity of the method and the results obtained in silico and with standard proteins, anticipate the utility of the SCAPE approach for proteome analyses.

引用

页码：491 / 496

页数：6

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