An electrochemical DNA biosensor developed on novel multinuclear nickel(II) salicylaldimine metallodendrimer platform

被引：32

作者：

Arotiba, Omotayo A. ^{[1
]}

Ignaszak, Anna ^{[1
]}

Malgas, Rehana ^{[2
]}

Al-Ahmed, Amir ^{[1
]}

Baker, Priscilla G. L. ^{[1
]}

Mapolie, Selwyn F. ^{[2
]}

Iwuoha, Emmanuel I. ^{[1
]}

机构：

[1] Univ Western Cape, Dept Chem, Sensor Res Lab, ZA-7535 Bellville, South Africa

[2] Univ Western Cape, Dept Chem, Organomet Res Grp, ZA-7535 Bellville, South Africa

来源：

ELECTROCHIMICA ACTA | 2007年 / 53卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

dendrimer; metallodendrimer; electrochemical DNA biosensor; immobilization; electrochemical impedance spectroscopy;

D O I：

10.1016/j.electacta.2007.08.016

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

An electrochemical DNA biosensor (EDB) was prepared using an oligonucleotide of 21 bases with sequence NH2-5'-GAGGAGTTGGGGGAGCACATT-3' (probe DNA) immobilized on a novel multinuclear nickel(II) salicylaldimine metallodendrimer on glassy carbon electrode (GCE). The metallodendrimer was synthesized from amino functionalized polypropylene imine dendrimer, DAB-(NH2)(8). The EDB was prepared by depositing probe DNA on a dendrimer-modified GCE surface and left to immobilize for 1 h. Voltammetric and electrochemical impedance spectroscopic (EIS) studies were carried out to characterize the novel metallodendrimer, the EDB and its hybridization response in PBS using [Fe(CN)(6)](3-/4-) as a redox probe at pH 7.2. The metallodendrimer was electroactive in PBS with two reversible redox couples at E degrees' = +200 mV and E degrees' = +434 mV; catalytic by reducing the E-pa of [Fe(CN)(6)](3-/4-) by 22 mV; conducting and has diffusion coefficient of 8.597 x 10(-1) cm(2) s(-1). From the EIS circuit fitting results, the EDB responded to 5 nM target DNA by exhibiting a decrease in charge transfer resistance (R-ct) in PBS and increase in R-ct in [Fe(CN)(6)](3-/4-) redox probe; while in voltammetry, increase in peak anodic current was observed in PBS after hybridization, thus giving the EDB a dual probe advantage. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：1689 / 1696

页数：8

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