The movement protein of Cowpea mosaic virus binds GTP and single-stranded nucleic acid in vitro

被引：17

作者：

Carvalho, CM

Pouwels, J

van Lent, JWM

Bisseling, T

Goldbach, RW

Wellink, J

机构：

[1] Univ Wageningen & Res Ctr, Virol Lab, Dept Plant Sci, NL-6709 PD Wageningen, Netherlands

[2] Univ Wageningen & Res Ctr, Dept Plant Sci, Mol Biol Lab, NL-6703 HA Wageningen, Netherlands

来源：

JOURNAL OF VIROLOGY | 2004年 / 78卷 / 03期

关键词：

D O I：

10.1128/JVI.78.3.1591-1594.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The movement protein (MP) of Cowpea mosaic virus forms tubules in plasmodesmata to enable the transport of mature virions. Here it is shown that the MP is capable of specifically binding riboguanosine triphosphate and that mutational analysis suggests that GTP binding plays a role in the targeted transport of the MP. Furthermore, the MP is capable of binding both single-stranded RNA and single-stranded DNA in a nonsequence-specific manner, and the GTP- and RNA-binding sites do not overlap.

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页码：1591 / 1594

页数：4

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