Accurate proteome-wide protein quantification from high-resolution 15N mass spectra

被引：15

作者：

Khan, Zia ^{[1
,7
]}

Amini, Sasan ^{[2
,7
]}

Bloom, Joshua S. ^{[2
,7
]}

Ruse, Cristian ^{[3
]}

Caudy, Amy A. ^{[7
]}

Kruglyak, Leonid ^{[4
,5
,7
]}

Singh, Mona ^{[1
,7
]}

Perlman, David H. ^{[2
,6
,7
]}

Tavazoie, Saeed ^{[2
,7
]}

机构：

[1] Princeton Univ, Dept Comp Sci, Princeton, NJ 08544 USA

[2] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA

[3] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

[4] Princeton Univ, Dept Ecol & Evolutionary Biol, Princeton, NJ 08544 USA

[5] Princeton Univ, Howard Hughes Med Inst, Princeton, NJ 08544 USA

[6] Princeton Univ, Princeton Mass Spectrometry Ctr, Princeton, NJ 08544 USA

[7] Princeton Univ, Lewis Sigler Inst Integrat Genom, Princeton, NJ 08544 USA

来源：

GENOME BIOLOGY | 2011年 / 12卷 / 12期

关键词：

ESCHERICHIA-COLI; STATIONARY-PHASE; GENE-EXPRESSION; IDENTIFICATION; QUANTITATION; CONVERSION;

D O I：

10.1186/gb-2011-12-12-r122

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

In quantitative mass spectrometry-based proteomics, the metabolic incorporation of a single source of N-15-labeled nitrogen has many advantages over using stable isotope-labeled amino acids. However, the lack of a robust computational framework for analyzing the resulting spectra has impeded wide use of this approach. We have addressed this challenge by introducing a new computational methodology for analyzing N-15 spectra in which quantification is integrated with identification. Application of this method to an Escherichia coli growth transition reveals significant improvement in quantification accuracy over previous methods.

引用

页数：10

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