Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV

被引：2283

作者：

Ou, Xiuyuan ^{[1
]}

Liu, Yan ^{[1
]}

Lei, Xiaobo ^{[1
]}

Li, Pei ^{[1
]}

Mi, Dan ^{[1
]}

Ren, Lili ^{[1
]}

Guo, Li ^{[1
]}

Guo, Ruixuan ^{[1
]}

Chen, Ting ^{[1
]}

Hu, Jiaxin ^{[1
]}

Xiang, Zichun ^{[1
]}

Mu, Zhixia ^{[1
]}

Chen, Xing ^{[2
]}

Chen, Jieyong ^{[3
]}

Hu, Keping ^{[2
]}

Jin, Qi ^{[1
]}

Wang, Jianwei ^{[1
]}

Qian, Zhaohui ^{[1
]}

机构：

[1] Chinese Acad Med Sci & Peking Union Med Coll, Inst Pathogen Biol, NHC Key Lab Syst Biol Pathogens, Beijing 100176, Peoples R China

[2] Chinese Acad Med Sci CAMS & Peking Union Med Coll, Inst Med Plant Dev IMPLAD, 151 Malianwa Rd North, Beijing 100193, Peoples R China

[3] Hengshui Third Peoples Hosp, Hebei, Peoples R China

来源：

NATURE COMMUNICATIONS | 2020年 / 11卷 / 01期

基金：

中国国家自然科学基金;

关键词：

RESPIRATORY SYNDROME-CORONAVIRUS; RECEPTOR-BINDING DOMAIN; HOST-CELL ENTRY; EBOLA-VIRUS; MERS-COV; FUNCTIONAL RECEPTOR; PROTEIN; ACTIVATION; IDENTIFICATION; CLEAVAGE;

D O I：

10.1038/s41467-020-15562-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Since 2002, beta coronaviruses (CoV) have caused three zoonotic outbreaks, SARS-CoV in 2002-2003, MERS-CoV in 2012, and the newly emerged SARS-CoV-2 in late 2019. However, little is currently known about the biology of SARS-CoV-2. Here, using SARS-CoV-2 S protein pseudovirus system, we confirm that human angiotensin converting enzyme 2 (hACE2) is the receptor for SARS-CoV-2, find that SARS-CoV-2 enters 293/hACE2 cells mainly through endocytosis, that PIKfyve, TPC2, and cathepsin L are critical for entry, and that SARS-CoV-2 S protein is less stable than SARS-CoV S. Polyclonal anti-SARS S1 antibodies T62 inhibit entry of SARS-CoV S but not SARS-CoV-2 S pseudovirions. Further studies using recovered SARS and COVID-19 patients' sera show limited cross-neutralization, suggesting that recovery from one infection might not protect against the other. Our results present potential targets for development of drugs and vaccines for SARS-CoV-2.

引用

页数：12

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