Functional expression of Cf9 and and Avr9 genes in Brassica napus induces enhanced resistance to Leptosphaeria maculans

被引:20
作者
Hennin, C
Höfte, M
Diederichsen, E
机构
[1] Aventis CropSci, B-9000 Ghent, Belgium
[2] Univ Ghent, Fac Agr & Appl Biol Sci, Phytopathol Lab, B-9000 Ghent, Belgium
关键词
defense responses; gene-for-gene concept; transgenic plants; two-component system;
D O I
10.1094/MPMI.2001.14.9.1075
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The tomato Cf9 resistance gene induces an Avr9-dependent hypersensitive response (HR) in tomato and transgenic Solanaceae spp. We studied whether the Cf9 gene product responded functionally to the corresponding Avr9 gene product when introduced in a heterologous plant species. We successfully expressed the Cf9 gene under control of its own promoter and the Avr9 or Avr9R8K genes under control of the p35S(1) promoter in transgenic oilseed rape. We demonstrated that the transgenic oilseed rape plants produced the Avr9 elicitor with the same specific necrosis-inducing activity as reported for Cladosporium fulvum. An Avr9-dependent HR was induced in Cf9 oilseed rape upon injection of intercellular fluid containing Avr9. We showed Avr9-specific induction of PR1, PR2, and Cxc750 defense genes in oilseed rape expressing Cf9. Cf9 x Avr9 oilseed rape did not result in seedling death of the F-1 progeny, independent of the promoters used to express the genes. The F-1 (Cf9 x Avr9) plants, however, were quantitatively more resistant to Leptosphaeria maculans. Phytopathological analyses revealed that disease development of L. maculans was delayed when the pathogen was applied on an Avr9-mediated HR site. We demonstrate that the Cf9 and Avr9 gene can be functionally expressed in a heterologous plant species and that the two components confer an increase in disease resistance.
引用
收藏
页码:1075 / 1085
页数:11
相关论文
共 47 条
[1]   The Rx gene from potato controls separate virus resistance and cell death responses [J].
Bendahmane, A ;
Kanyuka, K ;
Baulcombe, DC .
PLANT CELL, 1999, 11 (05) :781-791
[2]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3]   Salicylic acid is not required for Cf-2- and Cf-9-dependent resistance of tomato to Cladosporium fulvum [J].
Brading, PA ;
Hammond-Kosack, KE ;
Parr, A ;
Jones, JDG .
PLANT JOURNAL, 2000, 23 (03) :305-318
[4]   The Arabidopsis dnd1 "defense, no death" gene encodes a mutated cyclic nucleotide-gated ion channel [J].
Clough, SJ ;
Fengler, KA ;
Yu, IC ;
Lippok, B ;
Smith, RK ;
Bent, AF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (16) :9323-9328
[5]   Uncoupling resistance from cell death in the hypersensitive response of Nicotiana species to Cauliflower mosaic virus infection [J].
Cole, AB ;
Király, L ;
Ross, K ;
Scheolz, JE .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2001, 14 (01) :31-41
[6]   Relationship between localized acquired resistance (LAR) and the hypersensitive response (HR): HR is necessary for LAR to occur and salicylic acid is not sufficient to trigger LAR [J].
Costet, L ;
Cordelier, S ;
Dorey, S ;
Baillieul, F ;
Fritig, B ;
Kauffmann, S .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1999, 12 (08) :655-662
[7]   Early defence responses induced by AVR9 and mutant analogues in tobacco cell suspensions expressing the Cf-9 resistance gene [J].
De Jong, CF ;
Honée, G ;
Joosten, MHAJ ;
De Wit, PJGM .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 2000, 56 (04) :169-177
[8]   TRANSFORMATION OF BRASSICA-NAPUS AND BRASSICA-OLERACEA USING AGROBACTERIUM-TUMEFACIENS AND THE EXPRESSION OF THE BAR AND NEO GENES IN THE TRANSGENIC PLANTS [J].
DEBLOCK, M ;
DEBROUWER, D ;
TENNING, P .
PLANT PHYSIOLOGY, 1989, 91 (02) :694-701
[10]   EVIDENCE FOR THE OCCURRENCE OF RACE AND CULTIVAR-SPECIFIC ELICITORS OF NECROSIS IN INTER-CELLULAR FLUIDS OF COMPATIBLE INTERACTIONS OF CLADOSPORIUM-FULVUM AND TOMATO [J].
DEWIT, PJGM ;
SPIKMAN, G .
PHYSIOLOGICAL PLANT PATHOLOGY, 1982, 21 (01) :1-+