Functional expression of Cf9 and and Avr9 genes in Brassica napus induces enhanced resistance to Leptosphaeria maculans

被引:20
作者
Hennin, C
Höfte, M
Diederichsen, E
机构
[1] Aventis CropSci, B-9000 Ghent, Belgium
[2] Univ Ghent, Fac Agr & Appl Biol Sci, Phytopathol Lab, B-9000 Ghent, Belgium
关键词
defense responses; gene-for-gene concept; transgenic plants; two-component system;
D O I
10.1094/MPMI.2001.14.9.1075
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The tomato Cf9 resistance gene induces an Avr9-dependent hypersensitive response (HR) in tomato and transgenic Solanaceae spp. We studied whether the Cf9 gene product responded functionally to the corresponding Avr9 gene product when introduced in a heterologous plant species. We successfully expressed the Cf9 gene under control of its own promoter and the Avr9 or Avr9R8K genes under control of the p35S(1) promoter in transgenic oilseed rape. We demonstrated that the transgenic oilseed rape plants produced the Avr9 elicitor with the same specific necrosis-inducing activity as reported for Cladosporium fulvum. An Avr9-dependent HR was induced in Cf9 oilseed rape upon injection of intercellular fluid containing Avr9. We showed Avr9-specific induction of PR1, PR2, and Cxc750 defense genes in oilseed rape expressing Cf9. Cf9 x Avr9 oilseed rape did not result in seedling death of the F-1 progeny, independent of the promoters used to express the genes. The F-1 (Cf9 x Avr9) plants, however, were quantitatively more resistant to Leptosphaeria maculans. Phytopathological analyses revealed that disease development of L. maculans was delayed when the pathogen was applied on an Avr9-mediated HR site. We demonstrate that the Cf9 and Avr9 gene can be functionally expressed in a heterologous plant species and that the two components confer an increase in disease resistance.
引用
收藏
页码:1075 / 1085
页数:11
相关论文
共 47 条
[31]   Measurement of active oxygen species generated in planta in response to elicitor AVR9 of Cladosporium fulvum [J].
Lu, H ;
Higgins, VJ .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1998, 52 (01) :35-51
[32]   Involvement of reactive oxygen species, glutathione metabolism, and lipid peroxidation in the Cf-gene-dependent defense response of tomato cotyledons induced by race-specific elicitors of Cladosporium fulvum [J].
May, MJ ;
HammondKosack, KE ;
Jones, JDG .
PLANT PHYSIOLOGY, 1996, 110 (04) :1367-1379
[33]   IDENTIFICATION OF DNA-SEQUENCES REQUIRED FOR ACTIVITY OF THE CAULIFLOWER MOSAIC VIRUS-35S PROMOTER [J].
ODELL, JT ;
NAGY, F ;
CHUA, NH .
NATURE, 1985, 313 (6005) :810-812
[34]  
RASMUSSEN U, 1992, PLANTA, V187, P328, DOI 10.1007/BF00195656
[35]  
Sambrook J., 1989, MOL CLONING
[36]   Resistance response physiology and signal transduction [J].
Scheel, D .
CURRENT OPINION IN PLANT BIOLOGY, 1998, 1 (04) :305-310
[37]   Systemic acquired resistance [J].
Sticher, L ;
MauchMani, B ;
Metraux, JP .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 1997, 35 :235-270
[38]   INHIBITION OF FUNGAL DISEASE DEVELOPMENT IN PLANTS BY ENGINEERING CONTROLLED CELL-DEATH [J].
STRITTMATTER, G ;
JANSSENS, J ;
OPSOMER, C ;
BATTERMAN, J .
BIO-TECHNOLOGY, 1995, 13 (10) :1085-1089
[39]  
STUIVER M, 1998, ICPP98 C ED
[40]   Expression of the Bs2 pepper gene confers resistance to bacterial spot disease in tomato [J].
Tai, TH ;
Dahlbeck, D ;
Clark, ET ;
Gajiwala, P ;
Pasion, R ;
Whalen, MC ;
Stall, RE ;
Staskawicz, BJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (24) :14153-14158