CpG-matured murine plasmacytoid dendritic cells are capable of in vivo priming of functional CD8 T cell responses to endogenous but not exogenous antigens

被引:150
作者
Salio, M
Palmowski, MJ
Atzberger, A
Hermans, IF
Cerundolo, V
机构
[1] John Radcliffe Hosp, Weatherall Inst Mol Med, Canc Res Tumor Immunol Unit, Oxford OX3 9DS, England
[2] John Radcliffe Hosp, Weatherall Inst Mol Med, MRC, Hematol Unit, Oxford OX3 9DS, England
关键词
PDC; T cell priming; type IIFN; H-Y tetramers;
D O I
10.1084/jem.20031059
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Plasmacytoid dendritic cells (PDCs) are a unique leukocyte population capable of secreting high levels of type I interferon (IFN) in response to viruses and bacterial stimuli. In vitro experiments have shown that upon maturation, human and murine PDCs develop into potent immunostimulatory cells; however, their ability to prime an immune response in vivo remains to be addressed. We report that CpG-matured murine PDCs are capable of eliciting in naive mice antigen-specific CTLs against endogenous antigens as well as exogenous peptides, but not against an exogenous antigen. Type I IFN is not required for priming, as injection of CpG-matured PDCs into type I IFN receptor-deficient mice elicits functional CTL responses. Mature PDCs prime CTLs that secrete IFN-gamma and protect mice from a minor challenge. In contrast, miniature PDCs are unable to prime antigen-specific CTLs. However, mice injected with miniature PDCs are fully responsive to secondary antigenic challenges, suggesting that PDCs have not induced long-lasting tolerance via anergic or regulatory T cells. Our results underline the heterogeneity and plasticity of different antigen-presenting cells, and reveal an important role of mature PDCs in priming CD8 responses to endogenous antigens, in addition to their previously reported ability to modulate antiviral responses via type I IFN.
引用
收藏
页码:567 / 579
页数:13
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