Successful establishment of long-term bone marrow cultures in 103 patients with myelodysplastic syndromes

被引：25

作者：

Alvi, S ^{[1
]}

Shaher, A ^{[1
]}

Shetty, V ^{[1
]}

Henderson, B ^{[1
]}

Dangerfield, B ^{[1
]}

Zorat, F ^{[1
]}

Joshi, L ^{[1
]}

Anthwal, S ^{[1
]}

Lisak, L ^{[1
]}

Little, L ^{[1
]}

Gezer, S ^{[1
]}

Mundle, S ^{[1
]}

Reddy, PL ^{[1
]}

Allampallam, K ^{[1
]}

Huang, XK ^{[1
]}

Galili, N ^{[1
]}

Borok, RZ ^{[1
]}

Raza, A ^{[1
]}

机构：

[1] Rush Univ, Rush Canc Inst, Sect Myeloid Dis, MDS Ctr, Chicago, IL 60612 USA

来源：

LEUKEMIA RESEARCH | 2001年 / 25卷 / 11期

关键词：

long-term bone marrow biopsy cultures; stromal cells; myelodysplastic syndromes; apoptosis; transmission electron microscopy; functional studies of MDS stroma;

D O I：

10.1016/S0145-2126(01)00061-3

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We used bone marrow biopsies instead of mononuclear cells to maintain long-term cultures from 103 patients belonging to all five sub-categories of myelodysplastic syndromes (MDS), as well as 12 normal controls. By week 4, 30-50% confluency was reached and could be maintained for up to 12 weeks with 100% confluency. The four prominent cells were fibroblasts, macrophages, endothelial cells and adipocytes. Immunohistochemical and electron microscopic studies provided lineage confirmation. Normal hematopoiesis was well supported by MDS stroma. Neither the FAB nor cytogenetics was co-related with the potency of growth. MDS stroma appears to be both morphologically and functionally normal. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：941 / 954

页数：14

共 41 条

[1] Bone marrow stroma from refractory anemia of myelodysplastic syndrome is defective in its ability to support normal CD34-positive cell proliferation and differentiation in vitro [J].

Aizawa, S ;

Nakano, M ;

Iwase, O ;

Yaguchi, M ;

Hiramoto, M ;

Hoshi, H ;

Nabeshima, R ;

Shima, D ;

Handa, H ;

Toyama, K .

LEUKEMIA RESEARCH, 1999, 23 (03) :239-246

[2] Establishment of stromal cell line from an MDS RA patient which induced an apoptopic change in hematopoietic and leukemic cells in vitro [J].

Aizawa, S ;

Hiramoto, M ;

Hoshi, H ;

Toyama, K ;

Shima, D ;

Handa, H .

EXPERIMENTAL HEMATOLOGY, 2000, 28 (02) :148-155

[3]

Alvi S, 2000, BLOOD, V96, p359A

[4]

BENNETT JM, 1982, BRIT J HAEMATOL, V51, P189, DOI 10.1111/j.1365-2141.1982.tb08475.x

[5]

BLAZSEK I, 1995, EXP HEMATOL, V23, P309

[6]

CHANG J, 1989, CANCER CELL-MON REV, V1, P17

[7]

CHANG J, 1991, BAILLIERE CLIN HAEM, V3, P775

[8] APOPTOSIS IS A COMMON HISTOPATHOLOGICAL FINDING IN MYELODYSPLASIA - THE CORRELATE OF INEFFECTIVE HEMATOPOIESIS [J].

CLARK, DM ;

LAMPERT, IA .

LEUKEMIA & LYMPHOMA, 1990, 2 (06) :415-418

[9] FUNCTIONAL-STUDIES OF BONE-MARROW HEMATOPOIETIC AND STROMAL CELLS IN THE MYELODYSPLASTIC SYNDROME (MDS) [J].

COUTINHO, LH ;

GEARY, CG ;

CHANG, J ;

HARRISON, C ;

TESTA, NG .

BRITISH JOURNAL OF HAEMATOLOGY, 1990, 75 (01) :16-25

[10] Negative regulators of hemopoiesis and stroma function in patients with myelodysplastic syndrome [J].

Deeg, HJ ;

Beckham, C ;

Loken, MR ;

Bryant, E ;

Lesnikova, M ;

Shulman, HM ;

Gooley, T .

LEUKEMIA & LYMPHOMA, 2000, 37 (3-4) :405-+

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