A simple, sensitive and widely applicable ELISA for S100B:: Methodological features of the measurement of this glial protein

被引:115
作者
Leite, Marina Conch [1 ]
Galland, Fabiana [1 ]
Brolese, Giovana [2 ]
Guerra, Maria Cristina [1 ]
Bortolotto, Josiane Woutheres [1 ]
Freitas, Rodrigo [2 ]
Vielra de Almeida, Lucia Maria [1 ]
Gottfried, Carmem [1 ,2 ]
Goncalves, Carlos-Alberto [1 ,2 ]
机构
[1] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Bioquim, Programa Posgrad Bioquim, BR-90035003 Porto Alegre, RS, Brazil
[2] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Programa Posgrad Neurocencias, BR-90035003 Porto Alegre, RS, Brazil
关键词
adipocyte; astrocyte; brain injury; ELISA; oxidative stress; S100B protein;
D O I
10.1016/j.jneumeth.2007.11.021
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
S100B expression, particularly extracellular S100B, is used as a parameter of glial activation and/or death in several situations of brain injury. Several immunoassays for S100B measurement are available, which differ with regard to specificity, sensitivity, sample application, and, of course, economic costs. We standardized two protocols for S100B measurement (range between 1.9 pg and 10 ng/mL) in human and rat samples from brain and adipose tissues, blood serum, cerebrospinal fluid, urine and cell culture. Abundance and secretion of this protein in adipose tissue reinforces the caution about its origin in blood serum. Interestingly, S100B recognition was affected by the redox status of the protein. This aspect should be considered in S100B measurement, assuming that oxidized and reduced forms possibly coexist in vivo and the equilibrium can be modified by oxidative stress of physiological or pathological conditions or even by obtaining sample conditions. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:93 / 99
页数:7
相关论文
共 36 条
  • [1] Serum S100B and antioxidant enzymes in bipolar patients
    Andreazza, Ana Cristina
    Cassini, Carina
    Rosa, Adriane Ribeiro
    Leite, Marina Conch
    de Almeida, Lucia M. V.
    Nardin, Patricia
    Cunha, Angelo B. N.
    Cereser, Keila Maria
    Santin, Aida
    Gottfried, Carmem
    Salvador, Mirian
    Kapczinski, Flavio
    Goncalves, Carlos Alberto
    [J]. JOURNAL OF PSYCHIATRIC RESEARCH, 2007, 41 (06) : 523 - 529
  • [2] S100: a multigenic family of calcium-modulated proteins of the EF-hand type with intracellular and extracellular functional roles
    Donato, R
    [J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2001, 33 (07) : 637 - 668
  • [3] Intracellular and extracellular roles of s100 proteins
    Donato, R
    [J]. MICROSCOPY RESEARCH AND TECHNIQUE, 2003, 60 (06) : 540 - 551
  • [4] Peripheral detection of s100β during cardiothoracic surgery:: What are we really measuring?
    Fazio, V
    Bhudia, SK
    Marchi, N
    Aumayr, B
    Janigro, D
    [J]. ANNALS OF THORACIC SURGERY, 2004, 78 (01) : 46 - 53
  • [5] Human milk contains S100B protein
    Gazzolo, D
    Monego, G
    Corvino, V
    Bruschettini, M
    Bruschettini, P
    Zelano, G
    Michetti, F
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2003, 1619 (02): : 209 - 212
  • [6] Calcium-modulated proteins change their immunoreactivity in the presence of Ca2+: A study of antibody recognition in a dot immunoassay for calmodulin, calcineurin (beta-subunit), and S100B
    Goncalves, CA
    Gottfried, C
    Kommers, T
    Rodnight, R
    [J]. ANALYTICAL BIOCHEMISTRY, 1997, 253 (01) : 127 - 130
  • [7] Extracellular SI00B protein modulates ERK in astrocyte cultures
    Gonçalves, DS
    Lenz, G
    Karl, J
    Gonçalves, CA
    Rodnight, R
    [J]. NEUROREPORT, 2000, 11 (04) : 807 - 809
  • [8] Regulation of protein phosphorylation in astrocyte cultures by external calcium ions: specific effects on the phosphorylation of glial fibrillary acidic protein (GFAP), vimentin and heat shock protein 27 (HSP27)
    Gottfried, C
    Valentim, L
    Salbego, C
    Karl, J
    Wofchuk, ST
    Rodnight, R
    [J]. BRAIN RESEARCH, 1999, 833 (02) : 142 - 149
  • [9] A specific and sensitive ELISA for measuring S-100b in cerebrospinal fluid
    Green, AJE
    Keir, G
    Thompson, EJ
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1997, 205 (01) : 35 - 41
  • [10] S100B protein in clinical diagnostics: Assay specificity
    Heizmann, CW
    [J]. CLINICAL CHEMISTRY, 2004, 50 (01) : 249 - 251