Nitrite as the major source of nitric oxide production by Arabidopsis thaliana in response to Pseudomonas syringae

被引：172

作者：

Modolo, LV

Augusto, O

Almeida, IMG

Magalhaes, JR

Salgado, I

机构：

[1] Univ Estadual Campinas, Inst Biol, Dept Bioquim, BR-13083970 Campinas, SP, Brazil

[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05513970 Sao Paulo, Brazil

[3] Inst Biol, Lab Bacteriol Vegetal, BR-13001970 Campinas, SP, Brazil

[4] EMBRAPA, Empresa Brasileira Pesquisa Agropecuaria, BR-36038330 Juiz De Fora, MG, Brazil

来源：

FEBS LETTERS | 2005年 / 579卷 / 17期

基金：

巴西圣保罗研究基金会;

关键词：

electron paramagnetic resonance; mitochondria; nitrate reductase; nitric oxide; nitrite reductase; plant-pathogen interaction;

D O I：

10.1016/j.febslet.2005.05.078

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The origin of nitric oxide (,NO) in plants is unclear and an 'NO synthase (NOS)-like enzyme and nitrate reductase (NR) are claimed as potential sources. Here we used wild-type and NR-defective double mutant plants to investigate 'NO production in Arabidopsis thaliana in response to Pseudomonas syringae pv maculicola. NOS activity increased substantially in leaves inoculated with P. syringae. However, electron paramagnetic resonance experiments showed a much higher 'NO formation that was dependent on nitrite and mitochondrial electron transport rather than on arginine or nitrate. Overall, these results indicate that NOS, NR and a mitochondrial-dependent nitrite-reducing activity cooperate to produce 'NO during A. thaliana-P. syringae interaction. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3814 / 3820

页数：7

共 34 条

[11]

GARCIAMATA C, 2005, NITRIC OXIDE SIGNALI, P65

[12] Reduction of nitrite to nitric oxide catalyzed by xanthine oxidoreductase [J].