micro-tomography;
bone micro-architecture;
mineral density;
small animal imaging;
D O I:
10.1016/j.nima.2005.03.097
中图分类号:
TH7 [仪器、仪表];
学科分类号:
0804 ;
080401 ;
081102 ;
摘要:
Micro-tomographic imaging of bone micro-architecture is increasingly used on wild and transgenic mice to follow effects of diseases or therapeutics. Synchrotron radiation micro-tomography (SR micro-CT) provides quantitative images at very high spatial resolution but has mainly been used in vitro. The aim of this work was to show the feasibility of SR micro-CT for assessing bone mineral density and micro-architecture in vivo in mice. Imaging with a pixel size of 10 mu m was performed on beam line ID19 at the ESRF using a special mouse holder. Two strains of mice (C3H/HeJ and C57BL/6J) were used for the experiment. First tests were performed in order to optimize the imaging conditions with respect to dose. Then, six mice of each group were imaged at doses of 7 and 13 Gy (total scan time < 5 min). A similar volume of interest was selected in each image to perform quantitative analysis. The first results on bone volume and mineralization revealed significant differences between the strains as expected. Although there was no apparent damage, the imaged femurs will be further investigated by histology to evaluate the effect of irradiation on bone cells. In conclusion, SR micro-CT provides in vivo images with high signal-to-noise ratio (SNR), very short scan time and may be used in longitudinal studies. (c) 2005 Elsevier B.V. All rights reserved.
机构:Washington Univ, Sch Med, Alvin J Siteman Canc Ctr, St Louis, MO 63130 USA
Lewis, JS
Achilefu, S
论文数: 0引用数: 0
h-index: 0
机构:Washington Univ, Sch Med, Alvin J Siteman Canc Ctr, St Louis, MO 63130 USA
Achilefu, S
Garbow, JR
论文数: 0引用数: 0
h-index: 0
机构:Washington Univ, Sch Med, Alvin J Siteman Canc Ctr, St Louis, MO 63130 USA
Garbow, JR
Laforest, R
论文数: 0引用数: 0
h-index: 0
机构:Washington Univ, Sch Med, Alvin J Siteman Canc Ctr, St Louis, MO 63130 USA
Laforest, R
Welch, MJ
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Alvin J Siteman Canc Ctr, St Louis, MO 63130 USAWashington Univ, Sch Med, Alvin J Siteman Canc Ctr, St Louis, MO 63130 USA
机构:Washington Univ, Sch Med, Alvin J Siteman Canc Ctr, St Louis, MO 63130 USA
Lewis, JS
Achilefu, S
论文数: 0引用数: 0
h-index: 0
机构:Washington Univ, Sch Med, Alvin J Siteman Canc Ctr, St Louis, MO 63130 USA
Achilefu, S
Garbow, JR
论文数: 0引用数: 0
h-index: 0
机构:Washington Univ, Sch Med, Alvin J Siteman Canc Ctr, St Louis, MO 63130 USA
Garbow, JR
Laforest, R
论文数: 0引用数: 0
h-index: 0
机构:Washington Univ, Sch Med, Alvin J Siteman Canc Ctr, St Louis, MO 63130 USA
Laforest, R
Welch, MJ
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Alvin J Siteman Canc Ctr, St Louis, MO 63130 USAWashington Univ, Sch Med, Alvin J Siteman Canc Ctr, St Louis, MO 63130 USA