A method for the automatic characterization of bone architecture in 3D mice microtomographic images

被引:54
作者
Martín-Badosa, E
Elmoutaouakkil, A
Nuzzo, S
Amblard, D
Vico, L
Peyrin, F
机构
[1] European Synchrotron Radiat Facil, F-38043 Grenoble, France
[2] Univ Barcelona, Dept FAO, Lab Opt, E-08028 Barcelona, Spain
[3] CREATIS, CNRS, UMR 5515, F-69621 Villeurbanne, France
[4] CHU St Etienne, LBBTO, INSERM, Equipe Mixte E9901, F-42023 St Etienne, France
关键词
computed microtomography; bone microarchitecture; mice; osteoporosis; trabecular and cortical bone; 3D analysis; small animal imaging;
D O I
10.1016/S0895-6111(03)00031-4
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
We developed an automatic method to characterize mice bone architecture from three-dimensional (3D) microtomographic images. The distal metaphyses of the femur of mice were imaged using 3D synchrotron radiation microtomography at the European Synchrotron Radiation Facility (ID19) with a voxel size of 6.65 mum. Within each reconstructed volume, a region of interest was defined and trabecular and cortical bones were automatically separated. Then, 3D morphologic and topologic model-independent parameters quantifying the 3D bone architecture were computed in both regions. The technique was applied to study the response of the C57BL/6J@Ico strain of mice submitted to a model of bone loss by hind limb unloading produced by tail-suspension. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:447 / 458
页数:12
相关论文
共 20 条
  • [1] AMBLARD D, 1998, BONE, V23, pS510
  • [2] The visualization and evaluation of bone architecture in the rat using three-dimensional X-Ray microcomputed tomography
    Barbier, A
    Martel, C
    de Vernejoul, MC
    Tirode, F
    Nys, M
    Mocaer, G
    Morieux, C
    Murakami, H
    Lacheretz, F
    [J]. JOURNAL OF BONE AND MINERAL METABOLISM, 1999, 17 (01) : 37 - 44
  • [3] 3D COMPUTED X-RAY TOMOGRAPHY OF HUMAN CANCELLOUS BONE AT 8 MU-M SPATIAL AND 10(-4) ENERGY RESOLUTION
    BONSE, U
    BUSCH, F
    GUNNEWIG, O
    BECKMANN, F
    PAHL, R
    DELLING, G
    HAHN, M
    GRAEFF, W
    [J]. BONE AND MINERAL, 1994, 25 (01): : 25 - 38
  • [4] HIP-FRACTURES IN THE ELDERLY - A WORLDWIDE PROJECTION
    COOPER, C
    CAMPION, G
    MELTON, LJ
    [J]. OSTEOPOROSIS INTERNATIONAL, 1992, 2 (06) : 285 - 289
  • [5] THE DIRECT EXAMINATION OF 3-DIMENSIONAL BONE ARCHITECTURE INVITRO BY COMPUTED-TOMOGRAPHY
    FELDKAMP, LA
    GOLDSTEIN, SA
    PARFITT, AM
    JESION, G
    KLEEREKOPER, M
    [J]. JOURNAL OF BONE AND MINERAL RESEARCH, 1989, 4 (01) : 3 - 11
  • [6] A new method for the model-independent assessment of thickness in three-dimensional images
    Hildebrand, T
    Ruegsegger, P
    [J]. JOURNAL OF MICROSCOPY-OXFORD, 1997, 185 : 67 - 75
  • [7] Hildebrand TOR, 1997, Comput Methods Biomech Biomed Engin, V1, P15, DOI 10.1080/01495739708936692
  • [8] Applications of synchrotron microtomography in osteoporosis research
    Kinney, JH
    Haupt, DL
    Ladd, AJC
    [J]. DEVELOPMENTS IN X-RAY TOMOGRAPHY, 1997, 3149 : 64 - 68
  • [9] Comparison of structure extraction methods for in vivo trabecular bone measurements
    Laib, A
    Rüegsegger, P
    [J]. COMPUTERIZED MEDICAL IMAGING AND GRAPHICS, 1999, 23 (02) : 69 - 74
  • [10] 3D micro-computed tomography of trabecular and cortical bone architecture with application to a rat model of immobilisation osteoporosis
    Laib, A
    Barou, O
    Vico, L
    Lafage-Proust, MH
    Alexandre, C
    Rügsegger, P
    [J]. MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING, 2000, 38 (03) : 326 - 332