A method for the automatic characterization of bone architecture in 3D mice microtomographic images

被引：54

作者：

Martín-Badosa, E

Elmoutaouakkil, A

Nuzzo, S

Amblard, D

Vico, L

Peyrin, F

机构：

[1] European Synchrotron Radiat Facil, F-38043 Grenoble, France

[2] Univ Barcelona, Dept FAO, Lab Opt, E-08028 Barcelona, Spain

[3] CREATIS, CNRS, UMR 5515, F-69621 Villeurbanne, France

[4] CHU St Etienne, LBBTO, INSERM, Equipe Mixte E9901, F-42023 St Etienne, France

来源：

COMPUTERIZED MEDICAL IMAGING AND GRAPHICS | 2003年 / 27卷 / 06期

关键词：

computed microtomography; bone microarchitecture; mice; osteoporosis; trabecular and cortical bone; 3D analysis; small animal imaging;

D O I：

10.1016/S0895-6111(03)00031-4

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We developed an automatic method to characterize mice bone architecture from three-dimensional (3D) microtomographic images. The distal metaphyses of the femur of mice were imaged using 3D synchrotron radiation microtomography at the European Synchrotron Radiation Facility (ID19) with a voxel size of 6.65 mum. Within each reconstructed volume, a region of interest was defined and trabecular and cortical bones were automatically separated. Then, 3D morphologic and topologic model-independent parameters quantifying the 3D bone architecture were computed in both regions. The technique was applied to study the response of the C57BL/6J@Ico strain of mice submitted to a model of bone loss by hind limb unloading produced by tail-suspension. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：447 / 458

页数：12

共 20 条

[1] AMBLARD D, 1998, BONE, V23, pS510
[2] The visualization and evaluation of bone architecture in the rat using three-dimensional X-Ray microcomputed tomography
Barbier, A
Martel, C
de Vernejoul, MC
Tirode, F
Nys, M
Mocaer, G
Morieux, C
Murakami, H
Lacheretz, F
[J]. JOURNAL OF BONE AND MINERAL METABOLISM, 1999, 17 (01) : 37 - 44
[3] 3D COMPUTED X-RAY TOMOGRAPHY OF HUMAN CANCELLOUS BONE AT 8 MU-M SPATIAL AND 10(-4) ENERGY RESOLUTION
BONSE, U
BUSCH, F
GUNNEWIG, O
BECKMANN, F
PAHL, R
DELLING, G
HAHN, M
GRAEFF, W
[J]. BONE AND MINERAL, 1994, 25 (01): : 25 - 38
[4] HIP-FRACTURES IN THE ELDERLY - A WORLDWIDE PROJECTION
COOPER, C
CAMPION, G
MELTON, LJ
[J]. OSTEOPOROSIS INTERNATIONAL, 1992, 2 (06) : 285 - 289
[5] THE DIRECT EXAMINATION OF 3-DIMENSIONAL BONE ARCHITECTURE INVITRO BY COMPUTED-TOMOGRAPHY
FELDKAMP, LA
GOLDSTEIN, SA
PARFITT, AM
JESION, G
KLEEREKOPER, M
[J]. JOURNAL OF BONE AND MINERAL RESEARCH, 1989, 4 (01) : 3 - 11
[6] A new method for the model-independent assessment of thickness in three-dimensional images
Hildebrand, T
Ruegsegger, P
[J]. JOURNAL OF MICROSCOPY-OXFORD, 1997, 185 : 67 - 75
[7] Hildebrand TOR, 1997, Comput Methods Biomech Biomed Engin, V1, P15, DOI 10.1080/01495739708936692
[8] Applications of synchrotron microtomography in osteoporosis research
Kinney, JH
Haupt, DL
Ladd, AJC
[J]. DEVELOPMENTS IN X-RAY TOMOGRAPHY, 1997, 3149 : 64 - 68
[9] Comparison of structure extraction methods for in vivo trabecular bone measurements
Laib, A
Rüegsegger, P
[J]. COMPUTERIZED MEDICAL IMAGING AND GRAPHICS, 1999, 23 (02) : 69 - 74
[10] 3D micro-computed tomography of trabecular and cortical bone architecture with application to a rat model of immobilisation osteoporosis
Laib, A
Barou, O
Vico, L
Lafage-Proust, MH
Alexandre, C
Rügsegger, P
[J]. MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING, 2000, 38 (03) : 326 - 332

← 1 2 →