Purification and properties of a phytase from rye

被引：58

作者：

Greiner, R ^{[1
]}

Konietzny, U ^{[1
]}

Jany, KD ^{[1
]}

机构：

[1] Fed Res Ctr Nutr, Ctr Mol Biol, D-76131 Karlsruhe, Germany

来源：

JOURNAL OF FOOD BIOCHEMISTRY | 1998年 / 22卷 / 02期

关键词：

D O I：

10.1111/j.1745-4514.1998.tb00236.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phytase (myo-inositol hexakisphosphate phosphohydrolase) has been purified about 2,000-fold from ungerminated rye with a recovery of 6%. The enzyme behaves as a monomeric protein of a molecular mass of about 67 kDa. Optimal pH for the degradation of phytate has been found at pH 6.0 and 45C. Kinetic parameters for the hydrolysis of Na-phytate are K(M) 300 mu M and k(cat) 358 s(-1) at 35C and pH 6.0. The rye enzyme exhibits a broad affinity for various phosphorylated compounds and hydrolyses phytate in a stepwise manner; the pentakis- and tetrakisphosphate were identified as 1(1, 2, 3, 4, 5)P(5) and 1(2, 3, 4, 5)P(4). Consequently, this enzyme is a 6-phytase (EC 3.1.3.26).

引用

页码：143 / 161

页数：19

共 49 条

[21]

IRVING GCJ, 1980, INOSITOL PHOSPHATES, P85

[22] INOSITOL 1,3,4,6-TETRAKISPHOSPHATE MOBILIZES CALCIUM IN XENOPUS OOCYTES WITH HIGH POTENCY [J].

IVORRA, I ;

GIGG, R ;

IRVINE, RF ;

PARKER, I .

BIOCHEMICAL JOURNAL, 1991, 273 :317-321

[23]

KONIETZNY U, 1995, J FOOD BIOCHEM, V18, P165, DOI 10.1111/j.1745-4514.1994.tb00495.x

[24] PURIFICATION AND CHARACTERIZATION OF A PHYTASE (MYO-INOSITOL-HEXAKISPHOSPHATE PHOSPHOHYDROLASE) ACCUMULATED IN MAIZE (ZEA-MAYS) SEEDLINGS DURING GERMINATION [J].

LABOURE, AM ;

GAGNON, J ;

LESCURE, AM .

BIOCHEMICAL JOURNAL, 1993, 295 :413-419

[25] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[26] PHYTIC ACID METABOLISM IN LILY (LILIUM-LONGIFLORUM THUNB) POLLEN [J].

LIN, JJ ;

DICKINSON, DB ;

HO, THD .

PLANT PHYSIOLOGY, 1987, 83 (02) :408-413

[27] METABOLISM OF INOSITOL PHOSPHATES .8. PURIFICATION AND MODE OF ACTION OF PHYTASE FROM PHASEOLUS-AUREUS [J].

MAITI, IB ;

MAJUMDER, AL ;

BISWAS, BB .

PHYTOCHEMISTRY, 1974, 13 (07) :1047-1051

[28]

MIHAILOVIC M. L., 1965, Plant and Soil, V23, P117, DOI 10.1007/BF01349122

[29] The phytase subfamily of histidine acid phosphatases: Isolation of genes for two novel phytases from the fungi Aspergillus terreus and Myceliophthora thermophila [J].

Mitchell, DB ;

Vogel, K ;

Weimann, BJ ;

Pasamontes, L ;

vanLoon, APGM .

MICROBIOLOGY-SGM, 1997, 143 :245-252

[30]

MOLLEMAN A, 1991, J BIOL CHEM, V266, P4658

← 1 2 3 4 5 →