A functional eukaryotic promoter is contained within the first intron of the hGH-N coding region

被引：10

作者：

Kolb, AF

Günzburg, WH

Brem, G

Erfle, V

Salmons, B

机构：

[1] Univ Wurzburg, Inst Immunol & Virol, D-97070 Wurzburg, Germany

[2] Univ Munich, Inst Mol Anim Breeding, D-80539 Munich, Germany

[3] GSF, Res Ctr, Inst Mol Virol, Oberschleissheim, Germany

[4] Vet Univ Vienna, Inst Virol, A-1030 Vienna, Austria

[5] Vet Univ Vienna, Inst Genet & Anim Breeding, A-1030 Vienna, Austria

[6] Bavarian Nord Res Inst, Oberschleissheim, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 247卷 / 02期

关键词：

D O I：

10.1006/bbrc.1998.8782

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human growth hormone is frequently used as a reporter gene in studies addressing the regulation of eukaryotic promoters. Here we present evidence that the first intron of the hGH-N coding region contains a novel eukaryotic promoter which is able to direct the expression of hGH-N and luciferase reporter genes. The corresponding transcriptional initiation site is located downstream of the splice acceptor site of exon 2. This internal hGH-N promoter can interfere with the transcriptional control elements of a promoter linked to the hGH-N coding region and thereby complicate the interpretation of data obtained with hGH-N as a reporter gene. (C) 1998 Academic Press.

引用

页码：332 / 337

页数：6

共 23 条

[1] A PITUITARY-SPECIFIC TRANS-ACTING FACTOR CAN STIMULATE TRANSCRIPTION FROM THE GROWTH-HORMONE PROMOTER IN EXTRACTS OF NONEXPRESSING CELLS [J].