Resemblance and dissemblance of Arabidopsis type II peroxiredoxins:: Similar sequences for divergent gene expression, protein localization, and activity

被引:101
作者
Bréhélin, C
Meyer, EH
de Souris, JP
Bonnard, G
Meyer, Y
机构
[1] Univ Perpignan, CNRS, UMR 5096, Lab Genome & Dev Plantes, F-66860 Perpignan, France
[2] Univ Strasbourg 1, CNRS, Inst Biol Mol Plantes, F-67084 Strasbourg, France
关键词
D O I
10.1104/pp.103.022533
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The Arabidopsis type II peroxiredoxin (PRXII) family is composed of six different genes, five of which are expressed. On the basis of the nucleotide and protein sequences, we were able to define three subgroups among the PRXII family. The first subgroup is composed of AtPRXII-B, -C, and -D, which are highly similar and localized in the cytosol. AtPRXII-B is ubiquitously expressed. More striking is the specific expression of AtPRXII-C and AtPRXII-D localized in pollen. The second subgroup comprises the mitochondrial AtPRXII-F, the corresponding gene of which is expressed constitutively. We show that AtPRXII-E, belonging to the last subgroup, is expressed mostly in reproductive tissues and that its product is addressed to the plastid. By in vitro enzymatic experiments, we demonstrate that glutaredoxin is the electron donor of recombinant AtPRXII-B for peroxidase reaction, but the donors of AtPRXII-E and AtPRXII-F have still to be identified.
引用
收藏
页码:2045 / 2057
页数:13
相关论文
共 62 条
  • [11] Cloning and expression of a new isotype of the peroxiredoxin gene of Chinese cabbage and its comparison to 2Cys-peroxiredoxin isolated from the same plant
    Choi, YO
    Cheong, NE
    Lee, KO
    Jung, BG
    Hong, CH
    Jeong, JH
    Chi, YH
    Kim, K
    Cho, MJ
    Lee, SY
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 258 (03) : 768 - 771
  • [12] Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
    Clough, SJ
    Bent, AF
    [J]. PLANT JOURNAL, 1998, 16 (06) : 735 - 743
  • [13] Dual action of the active oxygen species during plant stress responses
    Dat, J
    Vandenabeele, S
    Vranová, E
    Van Montagu, M
    Inzé, D
    Van Breusegem, F
    [J]. CELLULAR AND MOLECULAR LIFE SCIENCES, 2000, 57 (05) : 779 - 795
  • [14] Devic M, 1997, PLANT PHYSIOL BIOCH, V35, P331
  • [15] The function of the chloroplast 2-cysteine peroxiredoxin in peroxide detoxification and its regulation
    Dietz, KJ
    Horling, F
    König, J
    Baier, M
    [J]. JOURNAL OF EXPERIMENTAL BOTANY, 2002, 53 (372) : 1321 - 1329
  • [16] Characterization of a plant mitochondrial active chromosome
    Fey, J
    Vermel, M
    Grienenberger, JM
    Maréchal-Drouard, L
    Gualberto, JM
    [J]. FEBS LETTERS, 1999, 458 (02) : 124 - 128
  • [17] EXHAUSTIVE MATCHING OF THE ENTIRE PROTEIN-SEQUENCE DATABASE
    GONNET, GH
    COHEN, MA
    BENNER, SA
    [J]. SCIENCE, 1992, 256 (5062) : 1443 - 1445
  • [18] Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii
    Goyer, A
    Haslekås, C
    Miginiac-Maslow, M
    Klein, U
    Le Marechal, P
    Jacquot, JP
    Decottignies, P
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 2002, 269 (01): : 272 - 282
  • [19] TOBACCO MESOPHYLL PROTOPLASTS SYNTHESIZE 1,3-BETA-GLUCANASE, CHITINASES, AND OSMOTINS DURING INVITRO CULTURE
    GROSSET, J
    MEYER, Y
    CHARTIER, Y
    KAUFFMANN, S
    LEGRAND, M
    FRITIG, B
    [J]. PLANT PHYSIOLOGY, 1990, 92 (02) : 520 - 527
  • [20] The expression of a peroxiredoxin antioxidant gene, AtPer1, in Arabidopsis thaliana is seed-specific and related to dormancy
    Haslekås, C
    Stacy, RAP
    Nygaard, V
    Culiáñez-Macià, FA
    Aalen, RB
    [J]. PLANT MOLECULAR BIOLOGY, 1998, 36 (06) : 833 - 845