Micropilot: automation of fluorescence microscopy-based imaging for systems biology

被引：73

作者：

Conrad, Christian ^{[1
]}

Wuensche, Annelie ^{[2
]}

Tan, Tze Heng ^{[2
]}

Bulkescher, Jutta ^{[1
]}

Sieckmann, Frank ^{[3
]}

Verissimo, Fatima ^{[2
]}

Edelstein, Arthur ^{[4
]}

Walter, Thomas ^{[2
]}

Liebel, Urban ^{[2
]}

Pepperkok, Rainer ^{[1
,2
]}

Ellenberg, Jan ^{[2
]}

机构：

[1] European Mol Biol Lab, Adv Light Microscopy Facil, Heidelberg, Germany

[2] European Mol Biol Lab, Cell Biol & Biophys Unit, Heidelberg, Germany

[3] Leica Microsyst GmbH, Mannheim, Germany

[4] Univ Calif San Francisco, Dept Cellular & Mol Pharmacol, San Francisco, CA 94143 USA

来源：

NATURE METHODS | 2011年 / 8卷 / 03期

关键词：

HETEROCHROMATIN; IDENTIFICATION; SELECTION; DYNAMICS;

D O I：

10.1038/NMETH.1558

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology.

引用

页码：246 / +

页数：6

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