Automated microscopy for high-content RNAi screening

被引：105

作者：

Conrad, Christian ^{[1
]}

Gerlich, Daniel W. ^{[2
]}

机构：

[1] European Mol Biol Lab Heidelberg, Adv Light Microscopy Core Facil, D-69117 Heidelberg, Germany

[2] ETH, Swiss Fed Inst Technol, Inst Biochem, CH-8093 Zurich, Switzerland

来源：

JOURNAL OF CELL BIOLOGY | 2010年 / 188卷 / 04期

基金：

瑞士国家科学基金会;

关键词：

QUANTITATIVE-ANALYSIS; REVERSE TRANSFECTION; CELL-DIVISION; OPEN TOOLS; INTERFERENCE; IDENTIFICATION; SYSTEMS; GENES; INFORMATICS; EXPLORATION;

D O I：

10.1083/jcb.200910105

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Fluorescence microscopy is one of the most powerful tools to investigate complex cellular processes such as cell division, cell motility, or intracellular trafficking. The availability of RNA interference (RNAi) technology and automated microscopy has opened the possibility to perform cellular imaging in functional genomics and other large-scale applications. Although imaging often dramatically increases the content of a screening assay, it poses new challenges to achieve accurate quantitative annotation and therefore needs to be carefully adjusted to the specific needs of individual screening applications. In this review, we discuss principles of assay design, large-scale RNAi, microscope automation, and computational data analysis. We highlight strategies for imaging-based RNAi screening adapted to different library and assay designs.

引用

页码：453 / 461

页数：9

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