Analysis of cell-based RNAi screens

被引：217

作者：

Boutros, Michael

Bras, Ligia P.

Huber, Wolfgang

机构：

[1] German Canc Res Ctr, D-69120 Heidelberg, Germany

[2] EMBL, European Bioinformat Inst, Cambridge CB10 1SD, England

[3] Univ Tecn Lisboa, IST, Ctr Chem & Biol Engn, P-1049001 Lisbon, Portugal

来源：

GENOME BIOLOGY | 2006年 / 7卷 / 07期

关键词：

D O I：

10.1186/gb-2006-7-7-r66

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw data. It is implemented in an open-source Bioconductor/R package, cellHTS (http://www.dkfz.de/signaling/cellHTS). The method is useful for the analysis and documentation of individual RNAi screens. Moreover, it is a prerequisite for the integration of multiple experiments.

引用

页数：11

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