Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth

被引:332
作者
Molendijk, AJ
Bischoff, F
Rajendrakumar, CSV
Friml, J
Braun, M
Gilroy, S
Palme, K
机构
[1] Max Planck Gesell, Max Delbruck Lab, D-50829 Cologne, Germany
[2] Max Planck Inst Zuchtungsforsch, D-50829 Cologne, Germany
[3] Univ Bonn, Inst Bot, D-53115 Bonn, Germany
[4] Penn State Univ, Dept Biol, Mueller Lab 208, University Pk, PA 16802 USA
关键词
Arabidopsis thaliana; polarity; Rho GTPase; root development; root hair;
D O I
10.1093/emboj/20.11.2779
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Plants contain a novel unique subfamily of Rho GTPases, vital components of cellular signalling networks. Here we report a general role for some members of this family in polarized plant growth processes. We show that Arabidopsis AtRop4 and AtRop6 encode functional GTPases with similar intrinsic GTP hydrolysis rates. We localized AtRop proteins in root meristem cells to the cross-wail and cell plate membranes. Polar localization of AtRops in trichoblasts specifies the growth sites for emerging root hairs. These sites were visible before budding and elongation of the Arabidopsis root hair when AtRops accumulated at their tips. Expression of constitutively active AtRop4 and AtRop6 mutant proteins in root hairs of transgenic Arabidopsis plants abolished polarized growth and delocalized the tip-focused Ca2+ gradient. Polar localization of AtRops was inhibited by brefeldin A, but not by other drugs such as latrunculin B, cytochalasin D or caffeine. Our results demonstrate a general function of AtRop GTPases in tip growth and in polar diffuse growth.
引用
收藏
页码:2779 / 2788
页数:10
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