Pathogen detection using short-RNA deep sequencing subtraction and assembly

被引：40

作者：

Isakov, Ofer ^{[1
]}

Modai, Shira ^{[1
]}

Shomron, Noam ^{[1
]}

机构：

[1] Tel Aviv Univ, Sackler Fac Med, Dept Cell & Dev Biol, IL-69978 Tel Aviv, Israel

来源：

BIOINFORMATICS | 2011年 / 27卷 / 15期

关键词：

DISCOVERY; VIRUSES;

D O I：

10.1093/bioinformatics/btr349

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Motivation: Early and accurate detection of human pathogen infection is critical for treatment and therapeutics. Here we describe pathogen identification using short RNA subtraction and assembly (SRSA), a detection method that overcomes the requirement of prior knowledge and culturing of pathogens, by using degraded small RNA and deep sequencing technology. We prove our approach's efficiency through identification of a combined viral and bacterial infection in human cells.

引用

页码：2027 / 2030

页数：4

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