Highly efficient DNA interstrand crosslinking induced by an antitumor antibiotic, carzinophilin

被引:53
作者
Fujiwara, T
Saito, I [1 ]
Sugiyama, H
机构
[1] Kyoto Univ, Fac Engn, Dept Synthet Chem & Biol Chem, Kyoto 6068501, Japan
[2] Tokyo Med & Dent Univ, Inst Med & Dent Engn, Tokyo 1010062, Japan
关键词
D O I
10.1016/S0040-4039(98)02339-9
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
The alkylation of d(TAGCTA)2 by an antitumor antibiotic, carzinophilin, and its 4-methyl derivative was investigated. It was found that both carzinophilin (1a) and 4-O-methylcarzinophilin (1b) react with d(TAGCTA)2 to provide the corresponding monoadducts and the interstrand crosslinked adducts. Heating the crosslinked adduct of Ib provided a stable base adduct which consists of a 1:1:1 ratio of adenine, guanine and 4-O-methylcarzinophilin. The structure of the base adduct was consistent with double crosslinks between guanine and the epoxide moiety and between adenine and the aziridine moiety. HPLC analysis indicates that the first alkylation occurs at the aziridine moiety with adenine N7 and the second crosslinking proceeds highly efficiently between the epoxide moiety and guanine N7. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:315 / 318
页数:4
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