Global mapping of the yeast genetic interaction network

被引：1509

作者：

Tong, AHY

Lesage, G

Bader, GD

Ding, HM

Xu, H

Xin, XF

Young, J

Berriz, GF

Brost, RL

Chang, M

Chen, YQ

Cheng, X

Chua, G

Friesen, H

Goldberg, DS

Haynes, J

Humphries, C

He, G

Hussein, S

Ke, LZ

Krogan, N

Li, ZJ

Levinson, JN

Lu, H

Ménard, P

Munyana, C

Parsons, AB

Ryan, O

Tonikian, R

Roberts, T

Sdicu, AM

Shapiro, J

Sheikh, B

Suter, B

Wong, SL

Zhang, LV

Zhu, HW

Burd, CG

Munro, S

Sander, C

Rine, J

Greenblatt, J

Peter, M

Bretscher, A

Bell, G

Roth, FP

Brown, GW

Andrews, B ^{[1
]}

Bussey, H

Boone, C

机构：

[1] Univ Toronto, Dept Med Genet & Microbiol, Toronto, ON M5S 1A8, Canada

[2] Univ Toronto, Banting & Best Dept Med Res, Toronto, ON M5G 1L6, Canada

[3] McGill Univ, Dept Biol, Montreal, PQ H3A 1B1, Canada

[4] Mem Sloan Kettering Canc Ctr, Computat Biol Ctr, New York, NY 10021 USA

[5] Univ Toronto, Dept Biochem, Toronto, ON M5S 1A8, Canada

[6] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA

[7] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

[8] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[9] Univ Penn, Sch Med, Dept Cell & Dev Biol, Philadelphia, PA 19104 USA

[10] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

[11] ETH Honggerberg, Inst Biochem, CH-8093 Zurich, Switzerland

来源：

SCIENCE | 2004年 / 303卷 / 5659期

关键词：

D O I：

10.1126/science.1091317

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A genetic interaction network containing similar to1000 genes and similar to4000 interactions was mapped by crossing mutations in 132 different query genes into a set of similar to4700 viable gene yeast deletion mutants and scoring the double mutant progeny for fitness defects. Network connectivity was predictive of function because interactions often occurred among functionally related genes, and similar patterns of interactions tended to identify components of the same pathway. The genetic network exhibited dense local neighborhoods; therefore, the position of a gene on a partially mapped network is predictive of other genetic interactions. Because digenic interactions are common in yeast, similar networks may underlie the complex genetics associated with inherited phenotypes in other organisms.

引用

页码：808 / 813

页数：6

共 25 条

[1] Mrc1 transduces signals of DNA replication stress to activate Rad53 [J].