Extended method for discrimination of Aeromonas spp. by 16S rDNA RFLP analysis

被引:92
作者
Figueras, MJ
Soler, L
Chacón, MR
Guarro, J
Martínez-Murcia, AJ
机构
[1] Univ Rovira & Virgili, Fac Med & Ciencias Salut, Dept Ciencias Med Basicas, Reus 43201, Spain
[2] Univ Miguel Hernandez, Div Microbiol, Alicante 03312, Spain
关键词
Aeromonas; 16S rDNA RFLP; molecular taxonomy;
D O I
10.1099/00207713-50-6-2069
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A previously described molecular method, based on 16S rDNA RFLP analysis, for the identification of Aeromonas spp. was unable to separate the species Aeromonas salmonicida, Aeromonas bestiarum and the recently described Aeromonas popoffii. In this study, the method has been extended with endonucleases AlwNI and Pstl for the identification of these species. A molecular frame for the identification of all known Aeromonas spp, is presented.
引用
收藏
页码:2069 / 2073
页数:5
相关论文
共 23 条
[1]   Misidentification of unusual Aeromonas species as members of the genus Vibrio:: A continuing problem [J].
Abbott, SL ;
Seli, LS ;
Catino, M ;
Hartley, MA ;
Janda, JM .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (04) :1103-1104
[2]  
Ali A, 1996, MED MICROBIOL LETT, V5, P156
[3]  
ASH C, 1993, FEMS MICROBIOL LETT, V108, P151, DOI 10.1016/0378-1097(93)90577-O
[4]  
ASH C, 1993, MED MICROBIOL LETT, V2, P80
[5]   Identification of Aeromonas clinical isolates by restriction fragment length polymorphism of PCR-amplified 16S rRNA genes [J].
Borrell, N ;
Acinas, SG ;
Figueras, MJ ;
MartinezMurcia, AJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (07) :1671-1674
[6]   Phenotypic identification of Aeromonas genomospecies from clinical and environmental sources [J].
Borrell, N ;
Figueras, MJ ;
Guarro, J .
CANADIAN JOURNAL OF MICROBIOLOGY, 1998, 44 (02) :103-108
[7]   Nomenclature for aerobic and facultative bacteria [J].
Bruckner, DA ;
Colonna, P ;
Bearson, BL .
CLINICAL INFECTIOUS DISEASES, 1999, 29 (04) :713-723
[8]  
CARNAHAN AM, 1996, GENUS AEROMONAS, P23
[9]   AEROMONAS-ENTEROPELOGENES AND AEROMONAS-ICHTHIOSMIA ARE IDENTICAL TO AEROMONAS-TROTA AND AEROMONAS-VERONII, RESPECTIVELY, AS REVEALED BY SMALL-SUBUNIT RIBOSOMAL-RNA SEQUENCE-ANALYSIS [J].
COLLINS, MD ;
MARTINEZMURCIA, AJ ;
CAI, J .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1993, 43 (04) :855-856
[10]   Signature region within the 16S rDNA sequences of Aeromonas popoffii [J].
Demarta, A ;
Tonolla, M ;
Caminada, AP ;
Ruggeri, N ;
Peduzzi, R .
FEMS MICROBIOLOGY LETTERS, 1999, 172 (02) :239-246