Extended method for discrimination of Aeromonas spp. by 16S rDNA RFLP analysis

被引:92
作者
Figueras, MJ
Soler, L
Chacón, MR
Guarro, J
Martínez-Murcia, AJ
机构
[1] Univ Rovira & Virgili, Fac Med & Ciencias Salut, Dept Ciencias Med Basicas, Reus 43201, Spain
[2] Univ Miguel Hernandez, Div Microbiol, Alicante 03312, Spain
关键词
Aeromonas; 16S rDNA RFLP; molecular taxonomy;
D O I
10.1099/00207713-50-6-2069
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A previously described molecular method, based on 16S rDNA RFLP analysis, for the identification of Aeromonas spp. was unable to separate the species Aeromonas salmonicida, Aeromonas bestiarum and the recently described Aeromonas popoffii. In this study, the method has been extended with endonucleases AlwNI and Pstl for the identification of these species. A molecular frame for the identification of all known Aeromonas spp, is presented.
引用
收藏
页码:2069 / 2073
页数:5
相关论文
共 23 条
[11]   RAPID IDENTIFICATION OF AEROMONAS SPECIES USING 16S RDNA TARGETED OLIGONUCLEOTIDE PRIMERS - A MOLECULAR APPROACH BASED ON SCREENING OF ENVIRONMENTAL ISOLATES [J].
DORSCH, M ;
ASHBOLT, NJ ;
COX, PT ;
GOODMAN, AE .
JOURNAL OF APPLIED BACTERIOLOGY, 1994, 77 (06) :722-726
[12]   Clinically relevant Aeromonas species [J].
Figueras, MJ ;
Guarro, J ;
Martínez-Murcia, A .
CLINICAL INFECTIOUS DISEASES, 2000, 30 (06) :988-989
[13]  
Figueras MJ, 2000, J CLIN MICROBIOL, V38, P2023
[14]   Diverse restriction fragment length polymorphism patterns of the PCR-amplified 16S rRNA genes in Aeromonas veronii strains and possible misidentification of Aeromonas species [J].
Graf, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (10) :3194-3197
[15]   AEROMONAS-SCHUBERTII, A NEW MANNITOL-NEGATIVE SPECIES FOUND IN HUMAN CLINICAL SPECIMENS [J].
HICKMANBRENNER, FW ;
FANNING, GR ;
ARDUINO, MJ ;
BRENNER, DJ ;
FARMER, JJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (08) :1561-1564
[16]   Inclusion of Aeromonas DNA hybridization group 11 in Aeromonas encheleia and extended descriptions of the species Aeromonas eucrenophila and A-encheleia [J].
Huys, G ;
Kampfer, P ;
Altwegg, M ;
Coopman, R ;
Janssen, P ;
Gillis, M ;
Kersters, K .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1997, 47 (04) :1157-1164
[17]   Aeromonas popoffii sp. nov., a mesophilic bacterium isolated from drinking water production plants and reservoirs [J].
Huys, G ;
Kampfer, P ;
Altwegg, M ;
Kersters, I ;
Lamb, A ;
Coopman, R ;
LuthyHottenstein, J ;
Vancanneyt, M ;
Janssen, P ;
Kersters, K .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1997, 47 (04) :1165-1171
[18]   Genotypic and chemotaxonomic description of two subgroups in the species Aeromonas eucrenophila and their affiliation to A-encheleia and Aeromonas DNA hybridization group 11 [J].
Huys, G ;
Altwegg, M ;
Hanninen, ML ;
Vancanneyt, M ;
Vauterin, L ;
Coopman, R ;
Torck, U ;
LuthyHottenstein, J ;
Janssen, P ;
Kersters, K .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1996, 19 (04) :616-623
[19]   Further studies on biochemical characteristics and serologic properties of the genus Aeromonas [J].
Janda, JM ;
Abbott, SL ;
Khashe, S ;
Kellogg, GH ;
Shimada, T .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (08) :1930-1933
[20]   Identification of Aeromonas trota (hybridization group 13) by amplification of the aerolysin gene using polymerase chain reaction [J].
Khan, AA ;
Nawaz, MS ;
Khan, SA ;
Cerniglia, CE .
MOLECULAR AND CELLULAR PROBES, 1999, 13 (02) :93-98